熵权-正交法优化赤苍藤中多糖、多酚和黄酮的提取工艺  

Optimization of multi-index extraction process of Erythropalum scandens BI.by entropy weight-orthogonal method

作　　者：陆玉婷[1,2,3,5] 毕明月 卓燊 李有娟[1,5] 陆苑 伍善广 LU Yuting;BI Mingyue;ZHUO Shen;LI Youjuan;LU Yuan;WU Shanguang(Medical School,Guangxi University of Science and Technology,Liuzhou 545005,China;School of Phar‐macy,Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Engineering Technology Re‐search Center of Bioactive Substance Discovery of Chinese Medicine,Changsha 410208,China;School of Pharmacy,Anhui Medical University,Hefei 230032,China;Liuzhou Key Laboratory of Guizhong Characteris‐tic Medicinal Resources Development(Guangxi University of Science and Technology),Liuzhou 545005,China)

机构地区：[1]广西科技大学医学部,广西柳州545005 [2]湖南中医药大学药学院,湖南长沙410208 [3]湖南省中药活性物质筛选工程技术研究中心,湖南长沙410208 [4]安徽医科大学药学院,安徽合肥230032 [5]柳州市桂中特色药用资源开发重点实验室(广西科技大学),广西柳州545005

出　　处：《广西科技大学学报》2024年第1期123-130,共8页Journal of Guangxi University of Science and Technology

基　　金：国家自然科学基金项目(21766003);广西高校中青年教师科研基础能力提升项目(2021KY0342)资助。

摘　　要：优化赤苍藤中多糖、多酚和黄酮的提取工艺,为综合评价其质量提供参考。以赤苍藤茎为研究对象,采用酶解法,选取适宜的酶质量分数、酶解p H、酶解温度及酶解时间为考察因素,以多糖、多酚、黄酮和浸膏提取率为评价指标。基于熵权法客观赋权结合L_9(3~4)正交试验,优选赤苍藤的多指标提取工艺。最佳提取工艺条件为:酶质量分数4%,酶解p H值6.0,酶解温度50℃,酶解时间45 min。考察多指标后,确认优选的提取工艺稳定可行,可为赤苍藤的质量评价提供实验依据。The extraction process of Erythropalum scandens Bl.was optimized to provide reference for comprehensive evaluation of its quality.The stem of E.Scandens was taken as the research object and the enzymatic method was used to select the appropriate enzyme dosage,enzymatic pH,enzymatic hydrolysis temperature and enzymatic hydrolysis time as the investigation factors,and extraction rates of polysaccharides,polyphenols,flavonoids and extracts were chosen as the evaluation indexes.Based on the entropy weight method and L9(34)orthogonal test,the multi-index extraction process was optimized.The optimum extraction conditions were as follows:enzyme mass fraction being 4%,enzymatic hydrolysis pH 6.0,enzymatic hydrolysis temperature 50℃,and enzymatic hydrolysis time 45 min.It is believed that the optimal extraction process is stable and feasible,which can provide experimental basis for the quality evaluation of Erythropalum scandens Bl.

关 键 词：赤苍藤 熵权法 酶解法 多指标评价 提取工艺 

分 类 号：R284.2[医药卫生—中药学]