STRAP binds to and promotes the repair of N1-methyldeoxyadenosine in DNA  

作　　者：Meijuan Zhou Qian Liu Mengting Pan Ying Yang Xiaoxia Dai Changjun You 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics,Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology,Molecular Science and Biomedicine Laboratory,College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China

出　　处：《Chinese Chemical Letters》2024年第3期204-207,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.21907028 and 21807030);Natural Science Foundation of Hunan Province(No.2023JJ30119);the Science and Technology Innovation Program of Hunan Province(No.2019RS2020);the Fundamental Research Funds for the Central Universities(Nos.531118010061 and 531118010259)。

摘　　要：N1-methyladenosine(m1A)is an important RNA modification that functions in various biological processes by interacting with cellular proteins.However,the binding proteins of N1-methyldeoxyadenosine(1mdA)in DNA remain largely unknown.Herein,we employed a quantitative proteomics strategy to identify the potential binding proteins of 1mdA in human cells.Our results revealed that serine–threonine kinase receptor-associated protein(STRAP)can bind to 1mdA-carrying DNA.We further demonstrated that STRAP participates in alkylating agent-induced DNA damage response and can promote the repair of 1mdA embedded in DNA.Moreover,we investigated the effects of STRAP on 1mdA-induced perturbation in transcription using a shuttle vector-and next-generation sequencing-based assay,and found that STRAP is involved in the transcriptional bypass of 1mdA in human cells.Together,our study revealed STRAP as a novel 1mdA-binding protein in human cells and provided new insight into the biological implications of STRAP and 1mdA modification in human diseases.

关 键 词：N1-methyldeoxyadenosine 1mdA-binding protein STRAP DNA repair TRANSCRIPTION 

分 类 号：Q75[生物学—分子生物学]