Continuous-flow enzymatic synthesis of chiral lactones in a three-dimensional microfluidic reactor  

作　　者：Xuelei Deng Meng Fan Miao Wu Xiaoyan Zhang Ya Cheng Jianye Xia Yingping Zhuang Weiping Zhu Xuhong Qian Yunpeng Bai 

机构地区：[1]State Key Laboratory of Bioreactor Engineering,Shanghai Collaborative Innovation Center for Biomanufacturing,East China University of Science and Technology,Shanghai 200237,China [2]The Extreme Optoelectromechanics Laboratory,School of Physics and Materials Science,East China Normal University,Shanghai 200241,China [3]Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China

出　　处：《Chinese Chemical Letters》2024年第3期299-303,共5页中国化学快报（英文版）

基　　金：financially sponsored by the National Key Research and Development Program of China(No.2021YFC2102804);the National Natural Science Foundation of China(No.22078096)。

摘　　要：A new continuous-flow process for the enzymatic synthesis of optically pureγ-lactones,which are used as flavors and fragrances in the food and cosmetic industries,was developed in a three-dimensional microfluidic reactor.The microchannels(175 mm in length,0.9 mm in depth,and 1.72 mL in volume)were carved precisely inside a single borosilicate glass(90 mm×75 mm×12 mm)with ultrafast femtosecond laser micromachining.The flow field analysis and reaction simulation showed that the mixing of substrates and enzymes was enhanced,allowing the adjustment of residence time in a wide window.SmCR_(V4),a carbonyl reductase with excellent catalytic activity and enantioselectivity towardγ/δ-keto acids,was employed for the asymmetric synthesis of various chiral lactones.30 mmol/L(R)-γ-decalactone(3g)can be obtained in 26 s with a space-time yield(STY)up to 16,877 g L^(-1)d^(-1),which is 14.4 times higher than the highest STY of batch reaction reported previously.This continuous-flow process was applied to the synthesis of 6 chiral lactones.In addition,the scaled-up synthesis of 3g was carried out in 6 cascade microreactors continuously for 6 h,demonstrating the feasibility and stability of the 3D continuous-flow process in enzymatic synthesis of optically pure compounds.

关 键 词：CONTINUOUS-FLOW Flow chemistry MICROREACTOR BIOCATALYSIS Carbonyl reductase Asymmetric reduction 

分 类 号：TQ235[化学工程—有机化工] TQ426.97