碳酸盐碱度胁迫对乌苏里白鲑幼鱼生长、抗氧化能力和生长基因表达的影响  

Effects of Carbonate Alkalinity Stress on Growth,Antioxidant Capacity and Expression of Growth-related Genes in Usscuri Whitefish Coregonus ussuriensis

作　　者：刘雪峰 黄天晴 刘恩慧 徐革锋[2] 史秀兰 董福霖 李文文 纪凯 谷伟[2] 潘玉财 王炳谦[2] LIU Xuefeng;HUANG Tianqing;LIU Enhui;XU Gefeng;SHI Xiulan;DONG Fulin;LI Wenwen;JI Kai;GU Wei;PAN Yucai;WANG Bingqian(School of Life Sciences and Technology,Harbin Normal University,Harbin 150025,China;Key Laboratory of Freshwater Aquatic Biotechnology and Breeding,Ministry of Agriculture and Rural Affairs,Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China)

机构地区：[1]哈尔滨师范大学生命科学与技术学院,黑龙江哈尔滨150025 [2]中国水产科学研究院黑龙江水产研究所,农业农村部淡水水产生物技术与遗传育种重点实验室,黑龙江哈尔滨150070

出　　处：《水产学杂志》2024年第1期1-8,共8页Chinese Journal of Fisheries

基　　金：中央级公益性科研院所基本科研业务费专项(HSY202103M);财政部和农业农村部国家现代农业产业技术体系资助(CARS-46);中国水产科学研究院科研业务费项目(2020TD32);农业财政专项“东北地区重点水域渔业资源与环境调查”.

摘　　要：为探究碱胁迫对乌苏里白鲑(Coregonus ussuriensis Berg)生长性能、抗氧化能力和胰岛素样生长因子-1(Insulin-like growth factor-1,IGF-1)及生长激素(Growth hormone,GH)基因表达的影响,在水温18℃下,将初始平均体质量(60.43±2.45)g的乌苏里白鲑饲养在180 cm×60 cm×47 cm的循环控温水族箱中6周,水中碳酸氢钠碱度为0 mmol/L、5 mmol/L、10 mmol/L、20 mmol/L和30 mmol/L,每个碱度组30尾鱼,三个平行组(n=10)。结果表明,在20mmol/L、30 mmol/L碱度下乌苏里白鲑的生长性能显著低于对照组(P<0.05),其存活率均为100%,而在10 mmol/L组中增重率(87.85%)与特定生长率(1.50%/d)最高,饲料系数(0.88)最低,生长性能显著高于其他组(P<0.05)。20 mmol/L、30 mmol/L碱度胁迫组中肝组织内超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH)和过氧化氢酶(CAT)活性及丙二醛(MDA)含量显著升高(P<0.05)。5 mmol/L、10 mmol/L碱度组中抗氧化酶活性与丙二醛含量与对照组无显著差异(P>0.05)。在5 mmol/L、10 mmol/L碱度下乌苏里白鲑IGF-1/GH生长轴基因的表达水平显著上升,10 mmol/L组表达量达峰值(P<0.05);20 mmol/L、30 mmol/L碳酸盐碱度下表达量显著下降,30 mmol/L组最低,仅为对照组的38%(P<0.05)和30%(P<0.05)。结果表明,乌苏里白鲑对碳酸盐碱度具有一定的耐受性,在10 mmol/L的低碱度环境中生长较好,尽管在20 mmol/L、30 mmol/L碱度不利于乌苏里白鲑生长,但其存活率仍为100%。To explore effects of alkalinity stress on Usscuri whitefish Coregonus ussuriensis Berg,the growth performance,antioxidant capacities and expressions of insulin-like growth factor-1(IGF-1)/growth hormone(GH) system genes were investigated in the Usscuri whitefish with an initial average body weight of(60.43 ±2.45) g reared in a circulating temperature-controlled aquarium of 180 cm×60 cm×47 cm at water temperature of 18 ℃ and exposed to sodium bicarbonate alkalinity of 0 mmol/L,5 mmol/L,10 mmol/L,20 mmol/L and 30 mmol/L in each alkalinity group,30 fish per alkalinity group,and three parallel groups(n=10) for 6 weeks.The results showed that the growth performance of C.ussuriensis was significantly decreased under the carbonate alkalinity 20 mmol/L and30 mmol/L compared with that in the control group(P < 0.05),with the survival rate of 100%.The maximal weight gain rate(87.85%)and specific growth ratio(1.50%),and the minimal feed conversion ratio(0.91) were observed at 10 mmol/L alkalinity(P < 0.05).The activities of glutathione(GSH),superoxide dismutase(SOD),and catalase(CAT) and malondialdehyde(MDA) content in liver tissue were found significantly increased in the 20 mmol/L and 30 mmol/L alkalinity group than those in the control group(P < 0.05),without significantly changes in the activities of the above three enzymes in the 5 mmol/L or 10 mmol/L alkalinity group(P > 0.05).The expression level of the IGF-1/GH system genes was found to be significantly up-regulated at alkalinity of 5 and 10 mmol/L(P < 0.05),andthe expression level of the IGF-1/GH system gene was significantly decreased at alkalinity of 20 mmol/L and 30 mmol/L(P < 0.05),with the minimum at 30 mmol/L.It was found that Usscuri whitefish had specific adaptability to 30 mmol/L alkalinity,and delivered better growth performance under the carbonate alkalinity 5 mmol/L and 10 mmol/L.These findings indicate that Usscuri whitefish has certain tolerances to carbonate alkalinity and has a good growth performance in the 10 mmol/L alkalinity,although the fi

关 键 词：乌苏里白鲑 碳酸盐碱度 生长性能 抗氧化能力 

分 类 号：S917.4[农业科学—水产科学]