壳三糖和壳五糖对睡眠剥夺小鼠学习记忆障碍的影响  

Effects of chitotriose and chitopentaose on learning and memory impairment in sleep-deprived mice

作　　者：王斌[1,2] 顾娟 闫华[1,2] 赵宁静 刘楠晖 张澎竹 姜启兴 许艳顺 夏文水[1,2] WANG Bin;GU Juan;YAN Hua;ZHAO Ningjing;LIU Nanhui;ZHANG Pengzhu;JIANG Qixing;XU Yanshun;XIA Wenshui(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province,Wuxi 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江苏省食品安全与质量控制协同创新中心,江苏无锡214122

出　　处：《食品与发酵工业》2024年第7期32-38,共7页Food and Fermentation Industries

基　　金：国家重点研发计划(蓝色粮仓科技创新)项目(2018YFD0901104)。

摘　　要：各种原因引起的睡眠时间减少已成为生活常态,睡眠剥夺会降低机体的学习记忆能力,影响生活质量。该研究对C57BL/6J小鼠进行21 d睡眠剥夺,同时每天对小鼠灌胃壳三糖(chitotriose,COS3)和壳五糖(chitopentaose,COS5),通过体重、新物体识别实验、病理学染色、氧化应激和凋亡相关蛋白表达评估COS3和COS5的保护作用。结果显示,COS3和COS5干预能够缓解小鼠体重下降和海马神经细胞坏死变形,显著提高海马组织中超氧化物歧化酶水平和总抗氧化能力,显著降低海马丙二醛含量。COS3和COS5干预能够显著提升海马p-PI3K(phospho-phosphotylinosital 3 kinase)和p-Akt(phospho-protein kinase B)蛋白的相对表达量,激活PI3K/Akt信号通路,缓解神经细胞凋亡。研究表明,COS3和COS5能够明显改善睡眠剥夺引起的学习记忆能力下降,其机制可能与COS3和COS5能够缓解海马组织氧化应激和神经细胞凋亡有关,其中COS3的效果优于COS5。Reduced sleep duration triggered by various factors has become a normal part of life.Sleep deprivation reduces the learning and memory capacity of the organism and affects the quality of life.In this study,C57BL/6J mice were subjected to sleep deprivation for 21 days,while chitotriose(COS3)and chitopentaose(COS5)were gavaged daily.The protective effects of COS3 and COS5 were assessed by weight,new object recognition assay,pathological staining,oxidative stress,and expression of the apoptosis-associated protein.Results showed that the intervention of COS3 and COS5 relieved the decrease in weight and necrosis and deformation of hippocampal neuronal cells.Additionally,the intervention of COS3 and COS5 improved the superoxide dismutase and total antioxidant capacity in the hippocampus and relieved malondialdehyde levels in the hippocampus.The intervention of COS3 and COS5 could significantly improve the relative expression of phospho-phosphotylinosital 3 kinase(p-PI3K)and phospho-protein kinase B(p-Akt)in the hippocampus,activate the PI3K/Akt signaling pathway and alleviate neuronal apoptosis.These results indicated that COS3 and COS5 had significant protective effects on the decline in learning memory capacity induced by sleep deprivation,with COS3 demonstrating a more significant effect compared to COS5.The mechanism may be related to the abilities of COS3 and COS5 to alleviate oxidative damage and neuronal apoptosis in the hippocampus of mice.

关 键 词：壳寡糖 睡眠剥夺 学习记忆 氧化应激 凋亡 

分 类 号：TS201.4[轻工技术与工程—食品科学]