饲用桑叶多糖提取工艺优化及其体外抗菌活性研究  

Optimization of forage mulberry leaf polysaccharide extraction process and its in vitro antimicrobial activity

作　　者：尚朝利 白泽方 樊轻亚[1] SHANG Chao-li;BAI Ze-fang;FAN Qing-ya

机构地区：[1]信阳职业技术学院,河南信阳464000

出　　处：《饲料研究》2024年第5期72-75,共4页Feed Research

基　　金：中共河南省委教育工委高校“双带头人”教师党支部书记工作室建设项目。

摘　　要：试验以饲用桑叶为原料,采用热水浸提法提取多糖。以提高桑叶中多糖提取率为优化目标,采用单因素试验分别研究液料比、提取温度和提取时间对多糖提取率的影响,利用正交分析法优化桑叶多糖提取工艺参数组合,并利用牛津杯法检测桑叶多糖的体外抑菌活性。结果显示,各因素对桑叶多糖提取率的影响排序为提取温度>液料比>提取时间,最优生产工艺参数组合为液料比30 L/g、提取温度90℃、提取时间3 h。在最优工艺条件下,桑叶多糖提取率为6.69%。桑叶多糖对金黄色葡萄球菌和大肠杆菌具有明显的抑制作用,抑菌圈直径分别为9.22、11.74 mm,最低抑菌浓度均为2.0 g/L。研究表明,热水浸提法提取的桑叶多糖可作为功能性饲料添加剂应用于动物养殖。In this experiment,polysaccharide was extracted from forage mulberry leaf by hot water extraction.To improve the extraction rate of polysaccharide in mulberry leaf,the effects of liquid-to-material ratio,extraction temperature,and extraction time on the polysaccharide extraction rate were studied by single factor experiment,combination of process parameters for the extraction of mulberry leaf polysaccharide was optimized by orthogonal analysis,and the antibacterial activity of mulberry leaf polysaccharide in vitro was detected by Oxford cup method.The results showed that the effects of various factors on the extraction rate of mulberry leaf polysaccharide were ranked as extraction temperature> liquid-to-material ratio > extraction time.The optimal combination of production process parameters was as follows:Liquid-material ratio of 30 L/g,extraction temperature of 90 ℃,and extraction time of 3 h.Under the optimal process conditions,the extraction rate of mulberry leaf polysaccharide was 6.69%.Mulberry leaf polysaccharide had obvious inhibitory effects on Staphylococcus aureus and Escherichia coli,with the diameter of the inhibition zone being 9.22 mm and 11.74 mm,respectively,and the lowest concentration of antibacterial was 2.0 g/L.The results showed that the mulberry leaf polysaccharide extracted by hot water extraction method could be used as functional feed additives in animal breeding.

关 键 词：饲用桑叶 多糖 热水浸提法 抑菌活性 

分 类 号：S816.7[农业科学—饲料科学]