铁沉积对非酒精性脂肪性肝炎兔模型肝脏质子密度脂肪分数的影响  

Impact of iron deposition on liver proton density fat fraction in non-alcoholic steatohepatitis rabbit models

作　　者：刘祝 肖如辉 赵艳芬 张治涛 杜勇[1] LIU Zhu;XIAO Ruhui;ZHAO Yanfen;ZHANG Zhitao;DU Yong(Department of Radiology,Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,China;Siemens Digital Medical TechnologyCo.,Ltd.Chengdu Branch,Chengdu 610095,China)

机构地区：[1]川北医学院附属医院放射科,四川南充637000 [2]西门子数字医疗科技(上海)有限公司成都分公司,四川成都610095

出　　处：《中国介入影像与治疗学》2024年第4期232-236,共5页Chinese Journal of Interventional Imaging and Therapy

基　　金：国家临床重点专科建设项目(川卫医政函[2023]87号)。

摘　　要：目的建立兔非酒精性脂肪性肝炎(NASH)模型,观察铁沉积对肝脏质子密度脂肪分数(PDFF)的影响。方法将70只新西兰大白兔随机分为单纯高脂组(A组,n=35)及高脂联合铁剂组(B组,n=35);再将B组分为1、2、3、4、8、12及16 ml共7个亚组,每组5只。对A组以高脂饲料饲养,对B组以高脂饲料饲养并经耳缘静脉注射右旋糖酐铁溶液(含铁100 mg/ml),建立NASH模型;行MRI及病理学检查,观察基于MRI所测PDFF与基于病理所测脂肪分数(FF)的相关性。结果建模结束时A、B组兔存活率分别为91.43%和74.29%,建模用时分别为(7.27±1.01)周和(5.07±1.27)周。B组各亚组中,随注射铁含量增加,MRI显示肝脏信号逐渐减低、胆囊及大血管显示欠清,其中1及2 ml亚组拟合优度分数均<5%、其余各亚组均≥5%,且仅1及2 ml亚组PDFF与FF相关(r=0.961、0.893,P=0.039、0.041)。结论NASH模型兔肝脏铁沉积增加将影响MRI测量肝脏PDFF的准确性。Objective To establish rabbit non-alcoholic steatohepatitis(NASH)model,and to observe the impact of iron deposition on liver proton density fat fraction(PDFF).Methods Seventy New Zealand white rabbits were randomly divided into simple high-fat group(group A,n=35)and high-fat combined iron group(group B,n=35),then those in group B were further divided into 7 subgroups(each n=5).NASH models were established through simply feeding with high-fat feed in group A,or combined with injecting iron dextran solution(containing 100 mg/ml iron)1,2,3,4,8,12 and 16 ml,respectively.MRI and pathological examinations were performed,and the correlation between PDFF measured with MRI and fat fraction(FF)measured with pathology was analyzed.Results After modeling,the survival rates of rabbits in group A and B was 91.43% and 74.29%,respectively,with modeling time of(7.27±1.01)and(5.07±1.27)weeks,respectively.In group B,with the increase of the iron content injection,the liver signal on MRI gradually decreased,and the gallbladder and large blood vessels became not clear,the goodness of fit scores of 1 and 2 ml subgroups were both<5%,while of the other 5 subgroups were≥5%.PDFF was correlated with FF in 1 and 2 ml subgroups(r=0.961,0.893;P=0.039,0.041).Conclusion The increased liver iron deposition could impact the accuracy of MRI measurement of PDFF in rabbit NASH models.

关 键 词：兔 非酒精性脂肪肝 脂肪组织 铁 磁共振成像 

分 类 号：R575.1[医药卫生—消化系统] R445.2[医药卫生—内科学]