华西牛与雪龙黑牛背最长肌重量的全基因组关联分析  

Genome-wide association analysis of longissimus dorsi weight between Huaxi cattles and Snow Dragon black cattles

作　　者：杜悦莹 安炳星 邓天宇 杜丽丽 梁忙 李柯安宁 曹晟 高会江[2] 闵令江[1] DU Yueying;AN Bingxing;DENG Tianyu;DU Lili;LIANG Mang;LI Keanning;CAO Sheng;GAO Huijiang;MIN Lingjiang(College of Animal Science and Technology,Qingdao agricultural university,Qingdao 266109,China;Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《黑龙江畜牧兽医》2024年第6期32-40,共9页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31872975);国家肉牛牦牛产业技术体系项目(CARS-37);财政部和农业农村部国家现代农业产业技术体系资助项目(CARS-37);内蒙古自治区科学技术计划项目(2020GG0210)。

摘　　要：为了筛选华西牛和雪龙黑牛背最长肌重量相关的遗传标记和候选基因,试验以1478头华西牛和452头雪龙黑牛为研究对象,对其背最长肌重量性状进行全基因组关联分析(genome-wide association study,GWAS),即测定其屠宰后背最长肌重量表型数据,利用Illumina Bovine-HD(770K)芯片对其基因组进行基因分型,采用FarmCPU模型和GEMMA模型对质控后的芯片数据进行GWAS,挖掘与华西牛和雪龙黑牛背最长肌重量显著相关的单核苷酸多态性(single nucleotide polymorphism,SNP)位点和候选基因。结果表明:华西牛与雪龙黑牛群体背最长肌重量表型性状均符合正态分布,且二者芯片数据经过质控后分别得到607198,511320个SNP位点,这些SNP位点在二者各个染色体上的分布比较均匀;利用FarmCPU模型在华西牛与雪龙黑牛群体分别检测到12个和9个与其背最长肌重量显著相关的SNP位点,且华西牛群体的显著SNP位点分别位于1,5,7,11,12,13,15,16,22,23号染色体上,鉴定到KCNAB1、PFN2、RNF13、TTLL1、MCAT、CREB3L3、ZBTB7A、MAP2K2、CAMKMT、ZC3H13、OPTN、MCM10、UCMA、OVOL2、PET117、KAT14、MAML2、HHAT、FHIT和LY86共20个候选基因;雪龙黑牛群体的SNP位点分别位于4,16,27号染色体上,鉴定到PCLO、DCTD、WWC2共3个候选基因;利用GEMMA模型在华西牛与雪龙黑牛群体中分别检测到6个与其背最长肌重量显著相关的SNP位点,其中华西牛群体的SNP位点位于13号染色体上,鉴定到GATA3、TAF3、ODAD2、SNX5、MGME1、OVOL2、KAT14、PET117共8个候选基因;雪龙黑牛群体的SNP位点位于1,4,5,25号染色体上,但未鉴定到有效的候选基因。说明在华西牛和雪龙黑牛群体中分别检测到的18个和15个SNP位点与注释到的25个和3个候选基因可作为改良牛背最长肌重量的新的分子标记和候选基因。In order to screen the genetic markers and candidate genes associated with the weight of the longissimus dorsi in Huaxi cattle and Snow Dragon black cattle, genome-wide association study(GWAS) was conducted on the weight traits of the longissimus dorsi in 1 478 Huaxi cattle and 452 Snow Dragon black cattle. The phenotypic data of longissimus dorsi muscle weight after slaughter were measured, and the genome was genotyped by Illumina Bovine-HD(770 K) chip. The GWAS was performed on the chip data after quality control using FarmCPU model and GEMMA model to explore the SNP loci and candidate genes significantly associated with the longissimus dorsi muscle weight of Huaxi cattle and Snow Dragon black cattle. The results showed that the phenotypic traits of longissimus dorsi muscle weight of Huaxi cattle and Snow Dragon black cattle were in accordance with the normal distribution. After quality control, 607 198 and 511 320 SNP loci were obtained from the microarray data of Huaxi cattle and Snow Dragon black cattle, respectively, and the distribution of these SNP loci was relatively uniform on each chromosome. By using the FarmCPU model, 12 and 9 SNP loci significant associated with the longissmus dorsi muscle weight were detected in Huaxi cattle and Snow Dragon black cattle populations, respectively, and the SNP loci in Huaxi cattle population were located on chromosomes 1, 5, 7, 11, 12, 13, 15, 16, 22, 23.Twenty candidate genes were identified, including KCNAB1, PFN2, RNF13, TTLL1, MCAT, CREB3L3, ZBTB7A, MAP2K2, CAMKMT, ZC3H13, OPTN, MCM10, UCMA, OVOL2, PET117, KAT14, MAML2, HHAT, FHIT and LY86. The SNP loci of Snow Dragon black cattle were located on chromosomes 4, 16, and 27, respectively, and three genes(PCLO, DCTD, and WWC2) were identified. Six SNP loci significant associated with the longissimus dorsi muscle weight were detected in both Huaxi cattle and Snow Dragon black cattle populations by GEMMA model. The SNP loci in Huaxi cattle population were located on chromosome 13, and GATA3, TAF3, ODAD2, SNX5, MGME1,

关 键 词：全基因组关联分析(GWAS) 华西牛 雪龙黑牛 背最长肌 重量 候选基因 

分 类 号：S823.92[农业科学—畜牧学]