铜藻岩藻多糖匀浆提取工艺及抗氧化活性分析  

Homogenization extraction process and analysis on antioxidant activities of fucoidan from Sargassum horneri

作　　者：蔡树芸 骆春萍 许敏 何袅袅 黄雅瑜 林米妮 张怡评 CAI Shuyun;LUO Chunping;XU Min;HE Niaoniao;HUANG Yayu;LIN Mini;ZHANG Yiping(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350108,China;Technical Innovation Center for Utilization of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resource,Xiamen 361005,China;School of Marine Biology,Xiamen Ocean Vocational College,Xiamen 361100,China;Fujian Provincial Key Laboratory of Island Conservation and Development,Fuzhou 350400,China)

机构地区：[1]福建中医药大学药学院,福建福州350108 [2]自然资源部第三海洋研究所海洋生物资源开发利用工程技术创新中心,福建厦门361005 [3]厦门海洋职业技术学院海洋生物学院,福建厦门361100 [4]福建省海岛资源生态监测与保护利用重点实验室,福建福州350400

出　　处：《药学研究》2024年第3期236-242,共7页Journal of Pharmaceutical Research

基　　金：厦门市海洋与渔业发展专项资金(No.23CZP009HJ11);自然资源部第三海洋研究所基本科研业务费专项资金资助项目(No.2022006);福建省科技计划项目(No.2021N0029);厦门市科技计划项目(No.3502Z20221036)。

摘　　要：目的优化铜藻岩藻多糖的匀浆提取工艺,并测定其抗氧化活性。方法以岩藻多糖的提取率为指标,基于单因素试验的基础上,采用响应面法对铜藻中岩藻多糖的提取工艺进行优化,并对其抗氧化活性进行验证。结果最佳提取工艺条件为提取时间6 min、提取温度94℃、液料比34 mL·g^(-1)和提取次数2次。在此条件下,多糖的提取率为14.76%。抗氧化活性研究结果表明,铜藻岩藻多糖在质量浓度为0.2~10.0 mg·mL^(-1)范围内有一定的抗氧化活性,对DPPH自由基、ABTS+自由基清除率的IC_(50)分别为0.22、0.41 mg·mL^(-1)。结论该提取工艺高效,可为铜藻岩藻多糖的制备及应用提供理论依据。Objective To optimize the homogenization extraction process of Sargassum horneri fucoidan and determine its antioxidant activity.Methods Based on the extraction yield of fucoidan and single factor experiments,response surface methodology was used to optimize the extraction process of fucoidan from Sargassum horneri,and its antioxidant activity was verified.Results The optimal extraction process conditions were extraction time of 6 min,extraction temperature of 94℃,liquid-solid ratio of 34 mg·mL^(-1),and extraction times of 2 times.Under these conditions,the yield of fucoidan was 14.76%.The results of antioxidant activity research indicate that Sargassum horneri fucoidan has certain antioxidant activity in the mass concentration range of 0.2~10.0 mg·mL^(-1),with IC 50 of 0.22 mg·mL^(-1)and 0.41 mg·mL^(-1)for DPPH and ABTS free radical scavenging rates,respectively.Conclusion The extraction process was efficient and can provide a theoretical basis for the preparation and application value of Sargassum horneri fucoidan.

关 键 词：铜藻岩藻多糖 匀浆法 提取工艺 响应面 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]