一种新型义齿材料的体外细胞毒性和家兔皮内反应试验研究  

The cytotoxicity in vitro and the intradermal reaction test in rabbits of a novel denture material

作　　者：王玉杰 陈洁雯 潘淑涛 范启明 区文诗 陈敏珊 黄红坤 张宏涛 陈芳[1] 李建军[1] WANG Yu-jie;CHEN Jie-wen;PAN Shu-tao;FAN Qi-ming;OU Wen-shi;CHEN Min-shan;HUANG Hong-kun;ZHANG Hong-tao;CHEN Fang;LI Jian-jun(School of Life Sciences and Engineering,Foshan University,Foshan,Guangdong 528000,China;Guangdong Zhongke Enhealth Technology Co.,Ltd,Foshan,Guangdong 528300,China)

机构地区：[1]佛山科学技术学院,广东佛山528000 [2]广东中科英海科技有限公司,广东佛山528300

出　　处：《毒理学杂志》2024年第1期51-56,共6页Journal of Toxicology

摘　　要：目的 通过体外细胞毒性和家兔皮内反应试验,对新型义齿材料的安全性进行初步评价。方法 JSAM-BL材料及2种3D打印试样分别用10%血清培养基、灭菌生理盐水和灭菌棉籽油浸提后,参考国家标准进行体外细胞毒性试验和皮内反应试验。结果 体外细胞毒性试验用10%血清培养基分别浸提JSAM-BL材料、JSAM-SLASH2-D牙冠和JSAM-TL3-D牙冠,3种试样100%浸提液的细胞生存率分别为2.40%、28.08%和73.92%(F=537.576,F=19.880,F=67.362,P<0.01)。家兔皮内反应试验中JSAM-BL材料、JSAM-SLASH2-D牙冠、JSAM-TL3-D牙冠极性试验组最终评分分别为0.20、0和0,非极性试验组最终评分分别为0、0和0.04。结论 体外细胞毒性试验结果表明,JSAM-BL材料、JSAM-SLASH2-D牙冠具有潜在细胞毒性,JSAM-TL3-D牙冠未显示有细胞毒性。家兔皮内反应试验表明,JSAM-BL材料、JSAM-SLASH2-D牙冠及JSAM-TL3-D牙冠的极性和非极性浸提液均无皮内反应。Objective A preliminary safety assessment of a novel denture material was conducted through in vitro cytotoxicity and intradermal reaction tests.Methods After maceration in 10%serum medium,sterilized saline,and sterilized cottonseed oil,respectively,the JSAM⁃BL material and two types of 3D⁃printed samples underwent in vitro cytotoxicity test and intradermal reaction test according to national standards.Results In the cytotoxicity test in vitro,JSAM⁃BL material,JSAM⁃SLASH2⁃D crown,and JSAM⁃TL3⁃D crown were macerated in 10%serum medium.The cell viability rates of the three samples in 100%maceration fluid were 2.40%,28.08%,and 73.92%,respectively.In the intradermal reaction test on rabbits,the polar test groups for JSAM⁃BL material,JSAM⁃SLASH2⁃D crown,and JSAM⁃TL3⁃D crown received final scores of 0.20,0,and 0,respectively,while the non⁃polar test groups received final scores of 0,0,and 0.04,respectively.Conclusion The result of the in vitro cytotoxicity test indicated potential cytotoxicity for JSAM⁃BL material and JSAM⁃SLASH2⁃D crown,while JSAM⁃TL3⁃D crown showed no cytotoxicity.The intradermal reaction test on rabbits demonstrated no dermal reactions for both polar and non⁃polar maceration fluids of JSAM⁃BL material,JSAM⁃SLASH2⁃D crown,and JSAM⁃TL3⁃D crown.

关 键 词：3D打印 牙冠 体外细胞毒性 MTT 家兔皮内反应 

分 类 号：R783.1[医药卫生—口腔医学] R99[医药卫生—临床医学] R114