依普黄酮促进人牙髓干细胞增殖和成骨分化  

Ipriflavone Promotes Proliferation and Mineralization of Human Dental Pulp Stem Cells

作　　者：乐曼妮 王小聪 黄子璇 张慧琳 张晓月 赵卿 李明[1,2] 王基栋 LE Manni;WANG Xiaocong;HUANG Zixuan;ZHANG Huilin;ZHANG Xiaoyue;ZHAO Qing;LI Ming;WANG Jidong(School of Stomatology,Hunan University of Traditional Chinese Medicine,Changsha 410208,China;Changsha Stomatological Hospital,Changsha 410004,China;Changde Hospital,Xiangya Medical College,Central South University,Changde 415000,China.)

机构地区：[1]湖南中医药大学口腔医(学)院,湖南长沙410208 [2]长沙市口腔医院,湖南长沙410004 [3]中南大学湘雅医学院附属常德医院,湖南常德415000

出　　处：《口腔医学研究》2024年第4期310-314,共5页Journal of Oral Science Research

基　　金：湖南省自然科学基金面上基金项目(编号:2022JJ30630);湖南省科技厅临床医疗技术创新引导项目(编号:2021SK53301);湖南省卫生健康委员会重点指导课题(编号:202108051626);湖南省教育厅重点项目(编号:22A0249);湖南省重点研发计划项目(编号:2020SK2137);长沙市自然科学基金(编号:kq2208458)。

摘　　要：目的:初步检测依普黄酮(IP)对人牙髓干细胞(human dental pulp stem cells,hDPSCs)的增殖和成骨向分化的影响。方法:在体外对hDPSCs进行培养鉴定,用含IP(10^(-9)~10^(-5) mol/L)的完全培养液培养hDPSCs,CCK-8法检测不同时间点(1、2、3 d)的细胞活性;用含IP(10-8~10^(-5) mol/L)的矿化诱导液诱导hDPSCs 7 d,通过碱性磷酸酶活性测定、ALP染色、茜素红染色及RT-qPCR检测IP对hDPSCs成骨分化的影响。结果:CCK-8检测结果表明10^(-9)~10^(-5) mol/L IP均可促进hDPSCs增殖,其中10^(-6) mol/L IP组促进增殖效果最佳(P<0.05);10^(-6) mol/L IP组ALP染色加深,ALP活性增高(P<0.05),矿化结节增多(P<0.05);RT-qPCR检测结果显示10^(-6) mol/L IP组能够提高成骨分化相关基因骨钙素(osteocalcin,OCN)、碱性磷酸酶和矮小相关转录基因2的表达水平(P<0.05)。结论:10^(-6) mol/L IP能提高hDPSCs增殖和成骨向分化的能力。Objective:To investigate the effects of ipriflavone(IP)on proliferation and mineralization of human dental pulp stem cells(hDPSCs).Methods:The hDPSCs were cultured in complete culture medium containing IP(10^(-9)-10^(-5) mol/L)and identified.The cell activity at different time points(1,2,3 d)was detected by CCK-8.After induced for 7 days with mineralization liquid containing IP(10-8-10^(-5) mol/L),the alkaline phosphatase(ALP)activity,ALP staining,alizarin red staining,and RT-qPCR were used to detect the osteogenic differentiation of hDPSCs.Results:CCK-8 detection showed that 10^(-9)-10^(-5) mol/L IP could promote the proliferation of hDPSCs,and 10^(-6) mol/L IP had the best results(P<0.05).In 10^(-6) mol/L IP group,the ALP staining was deepened,the activity was increased(P<0.05),and the mineralization nodules were increased.RT-qPCR showed that the contents of Runt-related transcription factor,ALP,and osteocalcin in the 10^(-6) mol/L IP group were significantly up-regulated(P<0.05).Conclusion:10^(-6) mol/L IP can promote the proliferation and mineralization of hDPSCs.

关 键 词：牙髓干细胞 增殖 成骨分化 依普黄酮 

分 类 号：R781.34[医药卫生—口腔医学]