晒田环境胁迫对克氏原螯虾卵巢组织、卵黄蛋白原及营养物质代谢的影响  

Effects of environmental stress of sunning field on ovarian tissue,vitellogenin and nutrient metabolism of Procambarus clarkii

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作  者:袁畅 李蔚 黄玉英 何苹萍 郑玉斯 李鑫 卢智发 张圣杰 李文红[2] 彭金霞 王大鹏 YUAN Chang;LI Wei;HUANG Yu-ying;HE Ping-ping;ZHENG Yu-si;LI Xin;LU Zhi-fa;ZHANG Sheng-jie;LI Wen-hong;PENG Jin-xia;WANG Da-peng(Guangxi Academy of Fishery Sciences/Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Breeding,Nanning 530021,China;College of Animal Science and Technology,Guangxi University,Nanning 530004,China)

机构地区:[1]广西水产科学研究院/广西水产遗传育种与健康养殖重点实验室,南宁530021 [2]广西大学动物科学技术学院,南宁530004

出  处:《西南农业学报》2024年第2期436-445,共10页Southwest China Journal of Agricultural Sciences

基  金:广西创新驱动发展专项(桂科AA20302019-3);广西农业农村厅科技自筹经费项目(Z2022100)。

摘  要:【目的】研究克氏原螯虾在晒田环境胁迫条件下卵巢组织学特点、卵黄蛋白原浓度及营养物质代谢水平的变化,探索其性成熟过程中体内营养物质变化相关机制,为晒田环境胁迫促进克氏原螯虾卵巢成熟这一生产操作的改良和标准化提供参考依据。【方法】试验对象分为晒田组与对照组克氏原螯虾,在晒田胁迫0、3、7、10 d时分别采集30只雌性克氏原螯虾,取卵巢进行石蜡切片和HE染色,并采集试验组的卵巢、肝胰腺和血清,检测卵黄蛋白原mRNA表达水平和浓度及营养物质代谢水平。【结果】雌性克氏原螯虾在晒田胁迫期间共鉴定出4个卵巢发育阶段,包括卵黄发生前阶段(Ⅰ期,卵巢为黄色,卵母细胞直径150~250μm)、初级卵黄发生阶段(Ⅱ期,卵巢为橙色,卵母细胞直径250~600μm)、次级卵黄发生阶段(Ⅲ期,卵巢为棕色,卵母细胞直径高于600μm)、成熟阶段(Ⅳ期,卵巢为黑褐色,卵母细胞直径高于1000μm)。晒田胁迫0 d时,晒田组与对照组雌性克氏原螯虾卵巢成熟率相似;晒田胁迫10 d时,晒田组雌性克氏原螯虾卵巢成熟率升至96.67%,而对照组仅升至60.00%。随着胁迫时间增加,晒田组和对照组雌性克氏原螯虾的卵巢与肝胰腺的卵黄蛋白原mRNA相对表达量均呈上升趋势,且卵黄蛋白原mRNA在肝胰腺的相对表达量大于卵巢;试验期间,晒田组和对照组雌性克氏原螯虾卵黄蛋白原在肝胰腺的表达总量均大于在卵巢的表达总量,且卵黄蛋白原在肝胰腺的贡献率均大于在卵巢的贡献率。晒田组与对照组雌性克氏原螯虾卵巢、肝胰腺和血液中的卵黄蛋白原浓度均随时间增加呈上升趋势;晒田组浓度上升幅度大于对照组,肝胰腺卵黄蛋白原浓度与卵巢卵黄蛋白原浓度、肝胰腺卵黄蛋白原浓度与血清卵黄蛋白原浓度、卵巢卵黄蛋白原浓度与血清卵黄蛋白原浓度呈极显著正相关(P<0.01)。随着晒田胁迫�【Objective】The present paper aimed to study the histological characteristics of the ovaries,the changes in concentration of vitello⁃genin and nutrient metabolism level of Procambarus clarkii under the stress of sunning field,explore the relevant mechanism of nutrient chan⁃ges during ovarian maturation process of P.clarkii,and the results provided a reference for the improvement and standardization of the pro⁃duction operation of P.clarkii ovary maturity which was promoted by environmental stress in sunning field.【Method】The experimental sub⁃jects were divided into sunning field group P.clarkii and control group P.clarkii.The female P.clarkii were collected at 0,3,7 and 10 days of the sunning field stress respectively.At each sampling time point,the ovaries were taken for paraffin section and HE staining.The ovaries,hepatopancreas and serum of samples were collected,of which the expression and concentration of vitellogenin mRNA and the level of nutrient metabolism were detected.【Result】Under the sunning field stress,four ovarian development stages were identified,including the pre⁃vitellogenesis stage(stage I,ovary was yellow,oocyte diameter 150-250μm),primary vitellogenesis stage(stage II,ovary was or⁃ange,oocyte diameter 250-600μm),secondary vitellogenesis stage(stage III,ovary was brown,oocyte diameter greater than 600μm),mature stage(stage IV,ovary was dark brown,oocyte diameter greater than 1000μm).At 0 days of the stress,the ovarian maturation rate of female P.clarkii in the sunning field group was similar to that in the control group.At 10 days of experiment,the ovarian maturation rate of the female P.clarkii in the sunning field group rose to 96.67%,while the control group rose to 60.00%.With stress time increasing,the relative expression of vitellogenin mRNA in the ovaries and hepatopancreas of the control group and the sunning field group increased,of which vitellogenin mRNA in the hepatopancreas was greater than that in the ovaries.Over the time of experiment,the total expres

关 键 词:克氏原螯虾 晒田环境胁迫 卵巢发育 卵黄蛋白原 营养物质代谢 卵巢组织学 

分 类 号:S963.7[农业科学—水产养殖]

 

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