茉莉酸甲酯对猕猴桃果实抗葡萄座腔菌过程中能量代谢和膜脂代谢的影响  

Effect of Methyl Jasmonate on Energy Metabolism and Membrane Lipid Metabolism During Resistance to Botryosphaeria dothidea in Kiwifruit

作　　者：肖刘华 康乃慧 李树成 郑致远 罗绕绕 陈金印[1] 陈明[1] 向妙莲[1] XIAO LiuHua;KANG NaiHui;LI ShuCheng;ZHENG ZhiYuan;LUO RaoRao;CHEN JinYin;CHEN Ming;XIANG MiaoLian(College of Agronomy,Jiangxi Agricultural University/Collaborative Innovation Center of Postharvest Key Technology and Quality Safety of Fruits and Vegetables in Jiangxi Province/Jiangxi Key Laboratory for Postharvest Technology and Non-Destructive Testing of Fruits&Vegetables,Nanchang 330045)

机构地区：[1]江西农业大学农学院/江西省果蔬采后处理关键技术与质量安全协同创新中心/江西省果蔬保鲜与无损检测重点实验室,南昌330045

出　　处：《中国农业科学》2024年第7期1377-1393,共17页Scientia Agricultura Sinica

基　　金：国家自然科学基金(32160399);江西省自然科学基金(20224BAB205031);江西省研究生创新专项基金(YC2022-s399,YC2023-B135)。

摘　　要：【背景】由葡萄座腔菌(Botryosphaeria dothidea)引起的软腐病是猕猴桃果实贮藏期的重要病害,对猕猴桃产业造成了严重的经济损失。茉莉酸甲酯(MeJA)是一类广泛存在于植物体内的生物信号分子,前期研究发现MeJA能够有效诱导猕猴桃果实抵御B.dothidea的侵染。【目的】分析MeJA对果实能量代谢和膜脂代谢的影响,深入解析MeJA介导的猕猴桃果实抗病机理。【方法】以‘红阳’猕猴桃(Actinidia chinensis cv.Hongyang)为试验材料,分3组进行试验:接种组(Inoculation),果实不经过MeJA处理,但接种B.dothidea;MeJA+接种组(MeJA+inoculation),果实经过0.1 mmol·L^(-1)MeJA熏蒸处理24 h并接种B.dothidea;对照组(Control),不作任何处理,即未经MeJA处理且不接种B.dothidea。所有果实于培养箱((20±1)℃、相对湿度90%—95%)中贮藏8 d。利用2,7-二氯二氢荧光素乙酰乙酸(H2DCFDA)染色分析果实活性氧(ROS)积累情况,测定丙二醛(MDA)含量和相对电导率,分析磷脂酶D(PLD)、脂肪酶(LPS)、脂氧合酶(LOX)、琥珀酸脱氢酶(SDH)、苹果酸脱氢酶(MDH)、H^(+)-ATP酶(H+-ATPase)、Ca^(2+)-ATP酶(Ca^(2+)-ATPase)和细胞色素C氧化酶(CCO)活性及其相应基因的表达,利用高效液相色谱仪(HPLC)检测三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、单磷酸腺苷(AMP)、草酰乙酸和延胡索酸的含量,并对接种组和MeJA+接种组猕猴桃果实膜脂代谢和能量代谢指标进行相关性分析。【结果】与接种组相比,MeJA处理抑制了接种B.dothidea果实中活性氧(ROS)的过量累积,减缓了膜脂过氧化程度,并有效降低了PLD、LPS和LOX活性及AcLOXs、AcPLD和AcLPS的表达,但提高了SDH、MDH、H^(+)-ATPase、Ca^(2+)-ATPase和CCO的活性及AcSDH、AcMDH、AcH^(+)-ATPase、AcCa^(2+)-ATPase和AcCCO转录水平,促进了ATP、ADP、草酰乙酸和延胡索酸的产生,延缓了果实能荷的下降,保障了果实抵御病原菌所需的能量。相关性分析表明,猕猴桃果实膜脂代谢与【Objective】Soft rot caused by Botryosphaeria dothidea is one of important diseases during kiwifruit storage,which causes serious economic losses for kiwifruit industry.Methyl jasmonate(MeJA)is a kind of biological signal molecule widely existing in plants,which had been found that it could effectively induce kiwifruit’s resistance to B.dothidea in previous studies.In order to further investigate the mechanism of MeJA-mediated resistance to disease in postharvest kiwifruit,the effects of MeJA on energy metabolism and membrane lipid metabolism were analyzed in this study.【Method】Hongyang kiwifruit(Actinidia chinensis cv.Hongyang)were used as experiment material and divided into three groups as follows:inoculation group,which the fruits were inoculated with B.dothidea without MeJA treatment;MeJA+inoculation group,which the fruits were fumigated with 0.1 mmol·L^(-1)MeJA for 24 h then inoculated with B.dothidea;control group,the fruits without MeJA treatment or inoculation with B.dothidea.All fruits were stored in an incubator((20±1)℃,90%-95%relative humidity)for 8 d.The reactive oxygen species(ROS)accumulation was analyzed by 2,7-dichlorodihydrofluorescein acetoacetate(H2DCFDA)method,malondialdehyde(MDA)content and relative conductivity were also measured.Meanwhile,phospholipase D(PLD),lipase(LPS),lipoxygenase(LOX),succinate dehydrogenase(SDH),malate dehydrogenase(MDH),H^(+)-ATPase,Ca^(2+)-ATPase and cytochrome C oxidase(CCO)activities and the related genes expression were analyzed.The content of Adenosine triphosphate(ATP),Adenosine diphosphate(ADP),Adenosine monophosphate(AMP),oxaloacetic acid and fenugreek acid were determined by high performance liquid chromatography(HPLC).Then the correlation between membrane lipid metabolism and energy metabolism of kiwifruit in inoculation group and MeJA+inoculation group was analyzed.【Result】Compared with the inoculated group,MeJA treatment could inhibit the excessive accumulation of ROS in inoculated B.dothidea fruits,slowed down the degree of membrane lipi

关 键 词：茉莉酸甲酯 猕猴桃果实 葡萄座腔菌 诱导抗性 能量代谢 膜脂代谢 

分 类 号：S436.634.1[农业科学—农业昆虫与害虫防治]