miR-93-5p/FOXJ2轴介导胃癌细胞的生物学功能  

miR-93-5p/FOXJ2 axis mediates biological function of gastric cancer cells

作　　者：沈蕾[1] 钮渊杰 宋巍 陈二林[3] SHEN Lei;NIU Yuanjie;SONG Wei;CHEN Erlin(Department of Laboratory Medicine,Affiliated Hospital of Nantong University,Jiangsu 226001;Medical College of Nantong University;Department of General Surgery,Affiliated Hospital of Nantong University,Jiangsu 226001)

机构地区：[1]南通大学附属医院医学检验科,江苏226001 [2]南通大学医学院 [3]南通大学附属医院普通外科,江苏226001

出　　处：《交通医学》2024年第1期1-5,10,共6页Medical Journal of Communications

基　　金：南通市科技计划指导性项目(JC22022010)。

摘　　要：目的:评估miR-93-5p对胃癌细胞的调节和功能。方法:采用qPCR检测miR-93-5p在胃癌组织、胃癌细胞系中表达,MTT、克隆形成、Transwell实验检测miR-93-5p对胃癌细胞增殖、侵袭的影响,双荧光素酶报告基因实验检测miR-93-5p与FOXJ2的靶向关系。结果:miR-93-5p在胃癌细胞系和胃癌组织中表达升高,抑制miR-93-5p表达后胃癌细胞增殖和侵袭能力受抑。双荧光素酶报告基因检测显示,miR-93-5p mimic显著降低MKN28和MKN45细胞Wt-FOXJ2相对荧光素酶活性,FOXJ2为胃癌细胞中miR-93-5p的直接靶基因。结论:miR-93-5p靶向FOXJ2促进胃癌细胞的增殖和侵袭,miR-93-5p/FOXJ2轴可能是判断胃癌预后的生物标志物和治疗靶点。Objective:To evaluate the regulation and function of miR-93-5p on gastric cancer cells.Methods:qPCR was used to detect the expression of miR-93-5p in gastric cancer tissue and gastric cancer cell lines.MTT,clone formation and Transwell were used to test the effect of miR-93-5p on proliferation and invasion of gastric cancer cells.Double luciferase reporter gene experiment was performed to test the targeting relationship between miR-93-5p and FOXJ2.Results:The expression of miR-93-5p was upregulated in gastric cancer cell lines and tissues,and the proliferation and invasion ability of gastric cancer cells was inhibited after inhibiting miR-93-5p expression.Double luciferase reporter gene detection showed that miR-93-5p mimic obviously decreased the relative luciferase activity of Wt-FOXJ2 in MKN28 and MKN45 cells,and FOXJ2 was the direct target gene of miR-93-5p.Conclusion:miR-93-5p targets FOXJ2 to promote the proliferation and invasion of gastric cancer cells,and miR-93-5p/FOXJ2 axis may be a biomarker and therapeutic target for the prognosis of gastric cancer.

关 键 词：胃癌 miR-93-5p 肿瘤标志物 增殖和侵袭 生物学功能 

分 类 号：R735.2[医药卫生—肿瘤]