Mechanism of acupuncture for limb spasm of post-stroke spasticity based on GABA and BDNF/Trkb-KCC2 signaling pathway  

作　　者：Qiong-Shuai Zhang Yi Li Yu Zhang Yu-Feng Wang Bai-Lin Song 

机构地区：[1]Department of Rehabilitation,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China. [2]Graduate School,Changchun University of Chinese Medicine,Changchun 130000,China. [3]College of Acupuncture-Moxibustion and Tuina,Changchun University of Chinese Medicine,Changchun 130117,China.

出　　处：《Traditional Medicine Research》2024年第7期42-48,共7页TMR传统医学研究

基　　金：the National Key R&D Program of China(Grant No.2019YFC1709900);National Natural Science Foundation of China(Grant No.71804022);Natural Science Foundation of Henan province(Grant No.232300420256);Medical technologies R&D Program of Henan province(Grant No.LHGJ20220348).

摘　　要：Background:To investigate the effects of acupuncture on post-stroke limb spasm model rats and the underlying mechanism.Methods:A total of 50 Sprague-Dawley rats were randomly divided into three groups,Control group(10),Model group(20)and Zhishen Tiaoxing(ZSTX)acupuncture group(20).Middle cerebral artery occlusion was conducted in SD rats to establish post-stroke limb spasm rats,which were treated with ZSTX acupuncture.Behavioral assays were determined by the Narrow ally test,the limb muscle tension was detected by the BL-420S test system,and infarct volume was assessed after the cerebral infarction by 2,3,5-triphenyltetrazolium chloride staining.Heterogeneous neurotransmitterγ-aminobutyric acid(GABA)and its receptors GABAA and GABAB in the cerebral cortex of the infarct area were determined by immunofluorescence assay.The release of Trkb and K-Cl cotransporter isoform 2 was detected by an immunofluorescence double labeling study.Western Blot was utilized to measure the expression of BDNF and Trkb.Results:The results showed that the behavioral assays in post-stroke limb spasm rats were significantly improved by the treatment of ZSTX acupuncture.14 days of ZSTX acupuncture can effectively inhibit muscle tone and decrease Infarct volume,which was measured with BL-420S biological function experiment system and triphenyltetrazolium chloride.Meanwhile,the results of Double-Label Immunofluorescence Assays showed that ZSTX acupuncture improved the expression of GABA,GABAA,GABAB,BDNF,and K-Cl cotransporter isoform 2.Double-Label Immunofluorescence Assays and WB results showed that 14 days ZSTX acupuncture declined the expression of Trkb.Conclusions:Our results suggest that 14 days of ZSTX acupuncture can significantly improve post-stroke limb spasm.Meanwhile,the pathogenesis of post-stroke limb spasm and the efficacy of ZSTX acupuncture involve metabolic pathways of neurotransmitters,and electro-acupuncture can treat post-stroke limb spasm by regulating BDNF/Trkb-KCC2 signaling pathway.

关 键 词：ACUPUNCTURE scalp acupuncture limb spasm of post-stroke 

分 类 号：R743.3[医药卫生—神经病学与精神病学]