急性髓系白血病患者血清lncRNA DANCR、lncRNA SNHG7表达及与病理特征、预后的关系  

Expressions of Serum LncRNA DANCR and LncRNA SNHG7 in Patients with Acute Myeloid Leukemia and Their Relationships with Pathological Characteristics and Prognosis

作　　者：邹安琪[1] 王悦 ZOU Anqi;WANG Yue(Department of Hematology,the First Affiliated Hospital of Harbin Medical University,Harbin 150001,China)

机构地区：[1]哈尔滨医科大学附属第一医院血液内科,黑龙江哈尔滨150001

出　　处：《标记免疫分析与临床》2024年第2期253-258,共6页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探究急性髓系白血病(AML)患者血清长链非编码RNA抗分化非编码RNA(lncRNA DANCR)、长链非编码RNA小核仁RNA宿主基因7(lncRNA SNHG7)表达及与病理特征、预后的关系。方法选取2017年10月至2018年10月期间在本院就诊后出院的120例AML患者记为AML组,另选取120例在本院体检的志愿者记为对照组。观察并记录所有参与者年龄、性别、AML形态学分型等临床指标。实时荧光定量PCR(qRT-PCR)方法检测lncRNA DANCR、lncRNA SNHG7表达量;t检验方法分析lncRNA DANCR、lncRNA SNHG7表达差异及其与病理特征的关系;Pearson相关性分析二者表达水平的相关性;根据二者表达水平均值将AML患者分为lncRNA DANCR高表达组(n=65)和lncRNA DANCR低表达组(n=55)及lncRNA SNHG7高表达组(n=74)和lncRNA SNHG7低表达组(n=46);Kaplan-Meier法进行生存分析。结果AML患者lncRNA DANCR、lncRNA SNHG7水平显著高于对照组(P<0.05),且二者具有正相关性(r=0.414,P<0.001);LncRNA DANCR、lncRNA SNHG7表达水平与危险度分层、白细胞(WBC)、血红蛋白(Hb)及血小板计数(PLT)相关(P<0.05);高表达lncRNA DANCR、lncRNA SNHG7的AML患者5年生存率分别为30.77%、31.08%,低表达lncRNA DANCR、lncRNA SNHG7的AML患者5年生存率分别为50.91%、54.35%(P<0.05),低表达lncRNA DANCR、lncRNA SNHG7的AML患者生存率显著更高(P<0.05)。结论LncRNA DANCR、lncRNA SNHG7在AML患者血清中表达水平较高,两者具有的正相关性,可作为AML患者预后不良的重要指标。Objective To investigate expressions of long non-coding RNA differentiation antagonizing non-protein coding RNA(lncRNA DANCR)and long non-coding RNA small nucleolar RNA host gene 7(lncRNA SNHG7)in serum of patients with acute myeloid leukemia(AML),and their relationships with pathological characteristics and prognosis.Methods In this study,120 patients with AML who were discharged from our hospital after visiting our clinic between October,2017 and October,2018 were selected and recorded as AML group,while another 120 volunteers who underwent physical examination at our hospital as the reference group.Clinical indicators,such as age,gender,and AML morphology were observed and recorded for all participants.Real-time fluorescence quantitative PCR(qRT-PCR)method was applied to detect the expression levels of lncRNA DANCR and lncRNA SNHG7;t-test method was applied to evaluate the differential expression of lncRNA DANCR and lncRNA SNHG7 and their relationships with pathological characteristics;Pearson correlation was applied to analyze the correlation between their expression levels;AML patients were grouped into lncRNA DANCR high expression group(n=65),lncRNA DANCR low expression group(n=55),lncRNA SNHG7 high expression group(n=74),and lncRNA SNHG7 low expression group(n=46)based on the mean expression levels of both;Kaplan-Meier method was applied for survival analysis.Results Levels of lncRNA DANCR and lncRNA SNHG7 in AML patients were significantly higher than those in the reference group(P<0.05),and there was a positive correlation between the two(r=0.414,P<0.001);Expression levels of LncRNA DANCR and lncRNA SNHG7 were correlated with risk stratification,white blood cell(WBC),hemoglobin(Hb),and platelet count(PLT)(P<0.05);The five-year survival rates of AML patients with high expression of lncRNA DANCR and lncRNA SNHG7 were 30.77%and 31.08%,respectively,while the five-year survival rates of AML patients with low expression of lncRNA DANCR and lncRNA SNHG7 were 50.91%and 54.35%,respectively(P<0.05),and the surviva

关 键 词：急性髓系白血病 长链非编码RNA抗分化非编码RNA 长链非编码RNA小核仁RNA宿主基因7 病理特征 预后 

分 类 号：R733.7[医药卫生—肿瘤]