Has_circ_0008717在羊膜间充质干细胞上清促血管生成中的作用研究  

The function of has_circ_0008717 in pro-angiogenic capacity of conditioned medium from human amnion-derived mesenchymal stem cells

作　　者：翟家彬[1] 袁乙钦 高洋[3] 陆史俊 钱文慧 唐子春 ZHAI Jiabin;YUAN Yiqin;GAO Yang;LU Shijun;QIAN Wenhui;TANG Zichun(Department of General Dentistry,Nanjing Stomatological Hospital,Affiliated Hospital of Medical School,Institute of Stomatology,Nanjing University,Nanjing 210029,China;Suzhou Medical College of Soochow University,Suzhou 215005,China;Department of Stomatology,The First Affiliated Hospital of Soochow University,Suzhou 215005,China;Department of Stomatology,Suzhou Municipal Hospital,Suzhou 215005,China;Department of Oral and Maxillofacial Surgery,The Affiliated Stomatological Hospital of Soochow University,Suzhou Stomatological Hospital,Suzhou 215005,China)

机构地区：[1]南京大学附属口腔医院·南京市口腔医院急诊综合科,南京大学口腔医学研究所,江苏南京210029 [2]苏州大学苏州医学院,江苏苏州215005 [3]苏州大学附属第一医院口腔科,江苏苏州215005 [4]苏州市立医院口腔科,江苏苏州215005 [5]苏州口腔医院口腔颌面外科,江苏苏州215005

出　　处：《口腔生物医学》2024年第2期91-96,共6页Oral Biomedicine

基　　金：苏州市科技发展计划项目(SKJY2021024,SKYD2023077);苏州市卫生人才项目(GSWS2022117);江苏省口腔疾病研究重点实验室开放课题(JSKLOD-KF-2305)。

摘　　要：目的:探究has_circ_0008717在人羊膜间充质干细胞上清(hAMSC-CM)促进人脐静脉内皮细胞(HUVECs)血管生成过程中的作用。方法:通过划痕实验、Transwell实验及成管实验检测hAMSC-CM对HUVECs迁移及成管能力的影响,实时荧光定量RT-PCR筛选上调的circRNA(has_circ_0008717);小干扰RNA(siRNA)下调HUVECs内has_circ_0008717水平后,实时荧光定量RT-PCR检测血管内皮生长因子A(VEGFA)表达水平变化,划痕实验、Transwell实验及成管实验观察其对hAMSC-CM促进HUVECs迁移及成管的影响。结果:hAMSC-CM可明显促进HUVECs迁移及成管能力(P<0.05),HUVECs中has_circ_0008717升高最为明显(P<0.05),而敲低has_circ_0008717可明显抑制hAMSC-CM促进HUVECs迁移、成管和VEGFA的表达(P<0.05)。结论:hAMSC-CM可通过has_circ_0008717增强HUVECs的成管能力。Objective:To investigate the role of has_circ_0008717 during the process that human amnion-derived mesenchymal stem cells(hAMSCs)promote angiogenesis of human umbilical vein endothelial cells(HUVECs).Methods:Wound healing assay,Transwell migration and tube formation were used to confirm the pro-angiogenic capacity of conditioned medium from hAMSCs(hAMSC-CM)on HUVECs,qRT-PCR and were used to detect the change of level of the potential pro-angiogenic relative circRNAs and genes in HUVECs after treated with hAMSC-CM,among them,has_circ_0008717 and vascular endothelial growth factor A(VEGFA)attracted our attention.siRNAs specially targeted has_circ_0008717 were used to down-regulate the level of has_circ_0008717 in HUVECs treated with hAMSC-CM,and then qRT-PCR was used to detect were used to evaluate the change of level of VEGFA caused by down-regulation of has_circ_00087,afterwards,wound healing assay,Transwell migration and tube formation were used to assess the effects of down-regulation of has_circ_0008717 on HUVECs treated with hAMSC-CM.Results:Enhanced migration and tube formation in HUVECs induced by hAMSC-CM were observed(P<0.05).And has_circ_0008717 been the mostly up-regulated circRNA(P<0.05).Increased migration,tube formation and expression of VEGFA was observed(P<0.05).Furthermore,these can be inhibited by silencing has_circ_0008717 in HUVEC treated with hAMSC-CM(P<0.05).Conclusion:has_circ_0008717 might be involved in the pro-angiogenic role of hAMSC-CM in HUVECs.

关 键 词：has_circ_0008717 羊膜间充质干细胞 血管生成 血管内皮生长因子A 

分 类 号：R780.2[医药卫生—口腔医学]