十二碳二元酸菌种发酵工艺优化  

Optimization of Fermentation Process of Dodecarbon Acid bacteria strains

作　　者：任勇 张会萍 张萍 牛春 Ren Yong;Zhang Hui-ping;Zhang Ping;Niu Chun(Ningxia Tairui Pharmaceutical Co.,LTD.,Yinchuan 750100)

机构地区：[1]宁夏泰瑞制药股份有限公司,银川750100

出　　处：《国外医药（抗生素分册）》2024年第2期109-114,共6页World Notes on Antibiotics

摘　　要：目的以热带假丝酵母菌为试验菌株,通过发酵工艺优化,提高菌种发酵产酸量。方法对发酵培养基中碳源和氮源等成分进行优化,同时研究了种龄、发酵培养温度、摇瓶转速、发酵接种量、发酵瓶装量和发酵pH值对发酵产酸量的影响。结果发酵培养最适碳源为2g/L蔗糖,最适氮源为3g/L酵母浸膏,菌体发酵在28℃,200r/min,25mL/250mL装量,以15%接种量条件下培养,发酵pH值调至7.0~7.5时,十二碳二元酸平均产量可达到130 g/L。结论对发酵培养基碳氮源成分进行优化、发酵工艺进行优化,发酵产酸量提高至130g/L。同时发酵培养基中添加氯化钠和硫酸镁后,均不利于菌种产酸。Objective To improve the acid production of Candida tropicalis by optimizing the fermentation process.Methods The effects of seed age,fermentation culture temperature,shake flask rotation speed,inoculation volume,filling volume,and sources for the 2 g/L of sucrose,the ideal nitrogen source for 3 g/L extract yeast,bacteria fermentation at 28℃,and fermentation pH value on acid production were also studied.The carbon source,nitrogen source,and other components in the fermentation medium were optimized.Results Fermentation to develop the optimal carbon 200 r/min,25 mL/250 mL bottle,with a 15%seed volume,under the condition of fermentation pH value to 7.0~7.5,the average yield of dodecarbonate can reach 130 g/L.Conclusion The carbon and nitrogen supply components of the fermentation medium were improved,along with the fermentation process,resulting in an increase in the fermentation acid production to 130 g/L.At the same time,The addition of sodium chloride and magnesium sulfate in the fermentation medium is not conducive to the production of acid by the strains.

关 键 词：热带假丝酵母菌 碳源 氮源 十二碳二元酸 优化 产酸量 

分 类 号：R978.1[医药卫生—药品]