电场对卷荚相思种子萌发和生理生化的影响  

Effects of electrical current on seed germination and physiological and biochemical properties in Acacia cincinnata

作　　者：朱梦恬 江瑞熠 李秉钧 邓智文 荣俊冬[1] 郑郁善[1] 陈礼光[1] ZHU Mengtian;JIANG Ruiyi;LI Bingjun;DENG Zhiwen;RONG Jundong;ZHENG Yushan;CHEN Liguang(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China)

机构地区：[1]福建农林大学林学院,福建福州350002

出　　处：《草业科学》2024年第3期709-717,共9页Pratacultural Science

基　　金：福建省科技厅重大专项(2018NZ0001-1)。

摘　　要：为了探明电场对卷荚相思(Acacia cincinnata)种子的生理响应,以卷荚相思种子为试验对象,设置相同时间(60 min)处理下不同电场强度(0.2、0.4、0.6、0.8、1.0 kV·cm^(−1))的试验组和对照组(CK,0 kV·cm^(−1)),研究电场处理对种子萌发和生理特性的影响。结果表明:卷荚相思种子的发芽势、发芽指数和发芽率在适宜的电场处理下与CK相比有一定程度的提高;相对电导率在电场强度0.8和0.6 kV·cm^(−1)下与CK相比显著降低(P<0.05);丙二醛含量在电场强度0.6 kV·cm^(−1)下与CK相比显著降低(P<0.05);酶活性在电场处理下呈不同变化趋势,过氧化物酶和超氧化物歧化酶活性随电场增强呈现先升后降的趋势,过氧化氢酶活性随着电场的升高呈现先下降后上升再下降的趋势。结构方程模型综合分析表明,电场处理对过氧化物酶和相对电导率有极显著(P<0.001)的影响,酶活性互相具有极显著的影响(P<0.001),丙二醛、过氧化物酶和过氧化氢酶对发芽率具有显著影响(P<0.01)。研究结果表明,适宜的电场处理对卷荚相思种子萌发和生理特性均产生显著影响,提高了种子的萌发效果。综上,适宜的电场处理可以促进种子的萌发,且电场强度0.6 kV·cm^(−1)处理下卷荚相思种子萌发可以达到萌发最佳效果。The effects of electricity on the seed germination and physiological characteristics of Acacia cincinnata were evaluated using different electric field intensities(0.2,0.4,0.6,0.8,and 1.0 kV·cm^(−1))for 60 min and compared to a control group(CK,0 kV·cm^(−1)).The results showed that the germination potential,germination index,and germination rate of A.cincinnata seeds were improved compared with CK:the relative conductivity was significantly lower than that of CK at 0.8 and 0.6 kV·cm^(−1)(P<0.05);the malonaldehyde(MDA)content was significantly lower than that of CK at 0.6 kV·cm^(−1)(P<0.05);the levels of peroxidase(POD)and superoxide dismutase(SOD)activity increased first and then decreased with an increase in the electric field,while the level of catalase(CAT)activity decreased first and then increased and decreased with an increase of electric field.A comprehensive analysis of the structural equation model showed that treatment with an electrical current had a significant effect on POD and relative conductivity(P<0.001).A significant effect was observed between enzyme activities(P<0.001),while MDA,POD,and CAT were found to have a significant effect on the germination rate.These results indicate that the application of a suitable electrical current has a significant effect on the germination and physiological characteristics of A.cincinnata seeds,as well as improving seed germination.In summary,treatment with an appropriate electrical current can promote seed germination.In particular,a voltage intensity of 0.6 kV·cm^(−1) achieved the best germination effect on A.cincinnata seeds.

关 键 词：电场处理 卷荚相思 种子萌发 结构方程 模型拟合 丙二醛 抗氧化酶 

分 类 号：S792.99[农业科学—林木遗传育种]