Cellulosomal hemicellulases:Indispensable players for ensuring effective lignocellulose bioconversion  

作　　者：Min Xiao Ya-Jun Liu Edward A.Bayer Akihiko Kosugi Qiu Cui Yingang Feng 

机构地区：[1]CAS Key Laboratory of Biofuels,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao 266101,China [2]Shandong Provincial Key Laboratory of Synthetic Biology,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao 266101,China [3]Shandong Engineering Laboratory of Single Cell Oil,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao 266101,China [4]Shandong Energy Institute,Qingdao 266101,China [5]Qingdao New Energy Shandong Laboratory,Qingdao 266101,China [6]University of Chinese Academy of Sciences,Beijing 100049,China [7]Department of Biomolecular Sciences,The Weizmann Institute of Science,Rehovot 7610001,Israel [8]Department of Life Sciences and the National Institute for Biotechnology in the Negev,Ben-Gurion University of the Negev,Beer-Sheva 8499000,Israel [9]Biological Resources and Post-harvest Division,Japan International Research Center for Agricultural Sciences(JIRCAS),1-1 Ohwashi,Tsukuba,Ibaraki 305-8686,Japan

出　　处：《Green Carbon》2024年第1期57-69,共13页绿碳（英文）

基　　金：supported by the National Natural Science Foundation of China[32070028 and 32370035 to Y.-J.L.,32070125 to Y.F.,and 32170051 to Q.C.];the National Key Research and Development Program of China[2021YFC2103600 to Y.-J.L.and 2023YFC3402304 to Y.F.];the International Partnership Program of Chinese Academy of Sciences[323GJHZ2022004MI to Y.-J.L.and A.K.];Shandong Energy Institute[SEI I202142 to Y.-J.L.,SEI S202106 to Q.C.,SEI I202106 to Y.F.];QIBEBT[Grant No.QIBEBT ICP2023046 to Y.F.].E.A.B.is the incumbent of The Maynard I.and Elaine Wishner Chair of Bio-organic Chemistry.

摘　　要：The bioconversion of lignocellulose has attracted global attention,due to the significant potential of agricultural and forestry wastes as renewable zero-carbon resources and the urgent need for substituting fossil carbon.The cellulosome system is a multi-enzyme complex produced by anaerobic bacteria,which comprises cellulases,hemicellulases,and associated enzymatic and non-enzymatic components that promote biomass conversion.To enhance their efficiency in degrading recalcitrant lignocellulosic matrices,cellulosomes have been employed to construct biocatalysts for lignocellulose bioconversion,such as consolidated bioprocessing and consolidated bio-saccharification.Hemicelluloses,the second most abundant polysaccharides in plant cell walls,hold valuable application potential but can also induce inhibitory effects on cellulose hydrolysis,thus highlighting the indispensable roles of hemicellulases within the cellulosome complex.This review evaluated current research on cellulosomal hemicellulases,comparing their types,abundance,and regulation,primarily focusing on eight known cellulosome-producing species of different origins.We also reviewed their growth conditions,their hemicellulose-degrading capabilities,and the inhibitory effects of hemicellulose on cellulosome-based lignocellulose saccharification.Finally,we proposed strategies for targeted enhancement of hemicellulase in cellulosomes to improve lignocellulose bioconversion in future studies.

关 键 词：CELLULOSOME LIGNOCELLULOSE HEMICELLULASE SACCHARIFICATION BIOCATALYST 

分 类 号：O64[理学—物理化学]