多星韭AwCHI 1的克隆与分析及真核表达载体的构建  

Cloning and analysis of chalcone isomerase gene from Allium wallichii and its eukaryotic expression vector construction

作　　者：赵鑫 张艳 肖松[1] 黄菊 陈瑶 孙威[1,2] ZHAO Xin;ZHANG Yan;XIAO Song;HUANG Ju;CHEN Yao;SUN Wei(School of Life Science/Key Laboratory of Plant Physiology and Development Regulation,Guizhou Normal University,Guiyang,Guizhou 550025,China;Key Laboratory of State Forestry Administration on Biodiversity Conservation in Karst Mountain Area of Southwest of China,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州师范大学生命科学学院/植物生理与发育调控重点实验室,贵州贵阳550025 [2]西南喀斯特山地生物多样性保护重点实验室,贵州贵阳550025

出　　处：《贵州师范大学学报（自然科学版）》2024年第2期118-126,共9页Journal of Guizhou Normal University：Natural Sciences

基　　金：贵州师范大学学术新苗基金项目([202]B04);喀斯特山地生态安全工程研究中心(黔教合KY字[2021]007);贵州省高等学校高山杜鹃病虫害绿色防控重点实验室项目(黔教技[2022]044号)。

摘　　要：查尔酮异构酶(Chalcone isomerase,CHI)是类黄酮物质合成途径中的关键酶,能催化柚皮素查尔酮生成柚皮素,进而转化生成各种类型的黄酮类化合物。根据多星韭(Allium wullichii)转录组测序结果,运用PCR技术克隆获取多星韭查尔酮异构酶的编码基因(AwCHI 1),对基因序列进行分析,同时与真核表达载体pBI121进行连接,将其转入农杆菌GV3101感受态细胞中,完成农杆菌的转化。研究结果不仅为该基因的功能解析研究奠定了基础,也为多星韭类黄酮代谢途径的研究提供了重要基因资源。Chalcone isomerase is a key enzyme in flavonoid synthesis pathway,which can catalyze the generation of naringenin from naringenin chalcone and convert it into various types of flavonoids.AwCHI 1 was successfully obtained from Allium wallichii by PCR based on the transcriptome data.Meanwhile,its sequence was analyzed by using bioinformatics method and subcloned into eukaryotic expression vector pBI121 which was transfered into the competent cells of Agrobacterium tumefaciens GV3101 to complete tromsformation.These results not only laid the foundation for the functional analysis of AwCHI 1,but also provided gene resources for the study of the synthesis pathway of flavonoids in A.wallichii.

关 键 词：类黄酮 查尔酮异构酶 多星韭 真核表达载体 

分 类 号：Q781[生物学—分子生物学] S682[农业科学—观赏园艺]