益母草碱对急性酒精性肝损伤的保护作用  

Protective effect of leonurine on acute alcohol-induced liver injury

作　　者：王炎 陈烁光 王娟[1] 陈旭[1] WANG Yan;CHEN Shuoguang;WANG Juan;CHEN Xu(College of Pharmacy,Guilin Medical University,Guilin 541199,China)

机构地区：[1]桂林医学院药学院,桂林541199

出　　处：《华夏医学》2024年第1期38-43,共6页Acta Medicinae Sinica

基　　金：广西科技基地和人才专项(桂科AD20238024);广西科技计划项目(桂科ZD20302006);第四批八桂学者2017年专项(2017-143)。

摘　　要：目的探究益母草碱对急性酒精性肝损伤的保护作用。方法将C57BL/6J小鼠随机分为对照组、模型组、益母草碱低剂量组、益母草碱高剂量组和联苯双酯组,每组8只。按确定药物与剂量给予益母草碱组和联苯双酯组灌胃,1次/日,连续10 d,对照组与模型组给予等体积注射用氯化钠溶液。末次给药12 h后,除对照组外,其余4组小鼠给予乙醇含量为56%的北京二锅头灌胃,连续3 d,每次间隔12 h。末次给予北京二锅头后,禁食4 h后处死小鼠,取肝脏称重,计算肝脏系数。检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、甘油三酯(TG)、丙二醛(MDA)含量。苏木素-伊红染色观察肝脏病理学变化,蛋白免疫印迹法检测相关蛋白。结果模型组小鼠ALT、AST、TG、MDA水平及肝脏指数显著高于对照组,差异有统计学意义(P<0.05);益母草碱低剂量组和高剂量组小鼠ALT、AST、TG、MDA水平及肝脏指数低于模型组(P<0.05)。肝脏组织病理检查及油红O染色揭示,益母草碱减轻乙醇对小鼠肝脏组织的损伤(P<0.05)。结论益母草碱对急性酒精性肝损伤具有保护作用。Objective To investigate the protective effects of leonurine against acute alcohol-induced liver injury.Methods The experimental design involved the random allocation of C57BL/6J mice into five groups:control group,model group,low-dose leonurine group,high-dose leonurine group,and Bifendate group,with 8 mice in each group.The leonurine and Bifendate groups were received their designatd doses through oral gavage once daily for a continuous period of 10 d.In contrast,the control and model groups were given an equivalent volume of saline solution during the same timeframe.12 h after the final dose,all groups except the control were given 56%alcohol content Beijing Erguotou by oral gavage for three consecutive days,with a 12-hour interval between each administration.After the final administration of Beijing Erguotou,the mice were fasted for 4 h before euthanasia,and their livers were weighed to calculate the liver coefficient.Serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglycerides(TG),and malondialdehyde(MDA)were measured.Hepatic pathological changes were observed via hematoxylin-eosin staining,and relevant proteins were detected by Western blot.Results In the model group,levels of ALT,AST,TG,MDA,and liver indices were significantly higher compared to the control group,with a statistically significant difference(P<0.05).The levels of ALT,AST,TG,MDA in both low-dose and high-dose leonurine groups were lower than those in the model group(P<0.05).Hepatic pathological examination and Oil Red O staining indicated that leonurine reduced ethanol-induced liver tissue damage in mice(P<0.05).Conclusion leonurine demonstrates a protective effect against acute alcohol-induced liver injury.

关 键 词：急性酒精性肝损伤 益母草碱 乙醇 肝损伤保护 

分 类 号：R575[医药卫生—消化系统]