Aptamer-based Membrane Protein Analysis and Molecular Diagnostics  

作　　者：ZHAO Long HU Haolan MA Xiaoqian LYU Yifan YUAN Quan TAN Weihong 

机构地区：[1]Molecular Science and Biomedicine Laboratory(MBL),State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Biology,College of Chemistry and Chemical Engineering,Aptamer Engineering Center of Hunan Province,Hunan University,Changsha,410082,P.R.China [2]The Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital),Hangzhou Institute of Medicine(HIM),Chinese Academy of Sciences,Hangzhou,310022,P.R.China [3]Institute of Molecular Medicine(IMM),Renji Hospital,School of Medicine,College of Chemistry and Chemical Engineering,Shanghai Jiao Tong University,Shanghai,200240,P.R.China [4]Furong Laboratory,Changsha,410082,P.R.China

出　　处：《Chemical Research in Chinese Universities》2024年第2期173-189,共17页高等学校化学研究（英文版）

基　　金：supported by the National Key Research and Development Project,China(No.2020YFA0909000);the National Natural Science Foundation of China(No.22107027);the Natural Science Foundation of Hunan Province,China(No.2023JJ20003);the Scientific Research Program of Furong Laboratory,China(No.2023SK2088).

摘　　要：Membrane proteins are vital components of the cell membrane and play crucial roles in various cellular activities.Analysis of membrane proteins is of paramount importance for studying molecular events inside cells and organisms and holds promising prospects for early disease diagnosis and treatment assessment.Benefiting from obvious merits including high affinity,high specificity and ease of modification,aptamers have been regarded as ideal molecular recognition elements in membrane protein analysis and molecular diagnostics strategies.This review summarised recent advances in membrane protein-specific aptamer screening,aptamer-based static and dynamic membrane protein analysis,and aptamer-based molecular diagnostic techniques.Prospects and challenges were also discussed.

关 键 词：Membrane protein APTAMER DNA nanotechnology BIOANALYSIS Molecular diagnostics 

分 类 号：Q51[生物学—生物化学]