Optimization on crystallization process of the magnetic core lysozyme  

作　　者：Lixia Hou Chunxiao Qu Min Su Zhihan Liang Qi Hao 

机构地区：[1]School of Chemical Engineering,Hebei University of Technology,Tianjin,300130,China

出　　处：《Particuology》2024年第4期106-113,共8页颗粒学报（英文版）

基　　金：supports from the National Natural Science Foundation of China (grant No.21878067)is greatly appreciated.

摘　　要：The continuous growth of the biomedical market highlights the significance of improvement in the ef-ficiency of batch production for protein drug crystals.Herein,we investigated the effect of polymer-modified Fe_(3)O_(4) nanoparticles on lysozyme(Lys)crystallization at different conditions,using function-alized Fe_(3)O_(4) magnetic nanoparticles(Polymer C@Fe_(3)O_(4))as nucleation agents.The study revealed that the surface charge density of Polymer C@Fe_(3)O_(4) was highest at a modification mass ratio of Fe_(3)O_(4) to Polymer C is 8∶3.This ratio facilitates the augmentation of electrostatic interaction and the promotion of crys-tallization.By adding 15%of the PolymerC@Fe_(3)O_(4) prepared from Fe_(3)O_(4) with an average particle size of 150 nm,the magnetic core lysozyme(M-Lys)crystals with an average particle size of 11.08μm,narrow size distribution,and regular morphology were obtained in a yield of 82.42%within 60 min.

关 键 词：PROTEIN CRYSTALLIZATION MAGNETIC PARTICLE LYSOZYME 

分 类 号：TG14[一般工业技术—材料科学与工程]