Chromosome-level assembly of triploid genome of Sichuan pepper(Zanthoxylum armatum)  

作　　者：Lizhi Song Yue Huang Hao Zuo Ning Tang Zhengguo Li Wenbiao Jiao Feng Xu Qiang Xu Zexiong Chen 

机构地区：[1]National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops,Huazhong Agricultural University,Wuhan,Hubei 430070,China [2]Chongqing Key Laboratory of Economic Plant Biotechnology,Chongqing University of Arts and Sciences,Chongqing 402160,China [3]School of Life Sciences,Chongqing University,Chongqing 400030,China [4]College of Horticulture and Gardening,Yangtze University,Jingzhou,Hubei 434025,China [5]College of Informatics,Huazhong Agricultural University,Wuhan,Hubei 430070,China

出　　处：《Horticultural Plant Journal》2024年第2期437-449,共13页园艺学报（英文版）

基　　金：supported by the Projects for Innovative Research Groups of Chongqing Universities (Grant No.CXQT21028);Chongqing talent program for Zexiong Chen;Scientific Technological Research Program of Chongqing Municipal Education Commission (Grant No.KJZD-K201901303);National Natural Science Foundation of China (Grant No.31925034);National Key Research and Development Project (Grant No.2019YFD1001200)。

摘　　要：As an important spice species in Rutaceae, the Sichuan pepper (Zanthoxylum armatum) can provide pungent and numbing taste, as well as aroma in its mature fruit. Here we assembled a chromosome-level genome of green prickly ash which was widely cultivated in a major production area including Chongqing and Sichuan province, China. We generated 712 Gb (~112×) PacBio long reads and 511 Gb (~82×) Hi-C data, and yielded an assembly of 99 pseudochromosomes with total size of 5.32 Gb and contig N50 of 796 kb. The genomic analyses and cytogenetic experiments both indicated that the cultivarZhuye Huajiao’ was a triploid. We identified a Zanthoxylum-specific whole genome duplication event emerging about 24.8 million years ago (Mya). We also detected a transposition burst event (0.3-0.4 Mya) responsible for the large genome size of Z. armatum. Metabolomic analysis of the Zanthoxylum fruits during development stages revealed profiles of39 volatile aroma compounds and 528 secondary metabolites, from which six types of sanshools were identified. Based on metabolomic and transcriptomic network analysis, we screened candidate genes encoding long chain acyl-CoA synthetase, fatty acid desaturase,branched-chain amino acid aminotransferase involved in sanshool biosynthesis and three genes encoding terpene synthase during fruit development. The multi-omics data provide insights into the evolution of Zanthoxylum and molecular basis of numbing and aroma flavor of Sichuan pepper.

关 键 词：Zanthoxylum armatum Genome Sanshool TERPENOID 

分 类 号：S573.9[农业科学—作物学]