3D printedβ-sheet-reinforced natural polymer hydrogel bilayer tissue engineering scaffold  

作　　者：ZHAO XinRui NIE XiongFeng ZHANG XiaoPing SUN YaGe YANG Rong BIAN XinYu ZHANG Qian WANG HongYing XU ZiYang LIU WenGuang 

机构地区：[1]School of Materials Science and Engineering,Tianjin Key Laboratory of Composite and Functional Materials,Tianjin University,Tianjin 300350,China

出　　处：《Science China(Technological Sciences)》2024年第4期1170-1184,共15页中国科学（技术科学英文版）

基　　金：supported by the National Key Research and Development Program of China(Grant No.2018YFA0703100)。

摘　　要：It remains a significant challenge to fabricate natural polymer(NP)hydrogels with anti-swelling ability and high strengths in the physiological environment.Herein,theβ-sheet-reinforced NP hydrogel is developed by copolymerizing methacrylated gelatin(GelMA)and methacrylated silk fibroin(SFMA)in aqueous solution,followed by ethanol treatment(named GelMA-SFMAAL).Theβ-sheets formed by SFMA can act as a stable physical crosslink to enhance the mechanical properties and prolong the degradation of the GelMA network.Importantly,the chemical crosslinking in the GelMA-SFMA hydrogel prevents excessive aggregation of hydrophobicβ-sheets,thereby avoiding the formation of brittle hydrogel.The obtained GelMA-SFMA-AL hydrogels exhibit considerably enhanced mechanical properties(Young's modulus:0.89–3.68 MPa;tensile strength:0.31–0.96 MPa;toughness:0.09–0.63 MJ/m^(3);compressive modulus:0.78–2.20 MPa;compressive strength:2.65–5.93 MPa)compared with GelMA-SFMA hydrogels(Young's modulus:0.04–0.13 MPa;tensile strength:0.04–0.07 MPa;toughness:0.01–0.02 MJ/m^(3);compressive modulus:0.03–0.09 MPa;compressive strength:0.30–0.64 MPa).A bilayer osteochondral scaffold is constructed via digital light processing(DLP)three-dimensiaonl(3D)printing technology,comprising GelMA-SFMA@diclofenac sodium(DS)-AL as the top layer and GelMA-SFMA@bioactive glass(BG)-AL as the bottom layer.The bilayer hydrogel scaffold is demonstrated to support cell attachment and spreading,and facilitate osteogenic differentiation of rat bone marrow stem cells in vitro.In vivo implantation experiment suggests this bilayer scaffold is promising to be used for osteochondral tissue regeneration.

关 键 词：silk fibroin Β-SHEET high-strength hydrogel natural polymer osteochondral regeneration 

分 类 号：TP391.73[自动化与计算机技术—计算机应用技术] TQ427.26[自动化与计算机技术—计算机科学与技术]