滑菇种质资源评价及遗传多样性分析  

Genetic Diversity Analysis of Pholiota nameko StrainsBased on ISSR and Agronomic Phenotype

作　　者：肇莹[1] 马晓颖[1] 陈珣[1] 龚娜[1] 刘国丽[1] 肖军[1] ZHAO Ying;MA Xiao-ying;CHEN Xun;GONG Na;LIU Guo-li;XIAO Jun(Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161)

机构地区：[1]辽宁省农业科学院,辽宁沈阳110161

出　　处：《辽宁农业科学》2024年第2期1-8,共8页Liaoning Agricultural Sciences

基　　金：辽宁省“揭榜挂帅”科技攻关项目(2021JH1/10400035)。

摘　　要：以26株滑菇菌株为试验材料,采用ISSR分子标记技术,利用筛选到的11条ISSR-PCR反应引物从细胞学水平和分子水平对滑菇的种质资源进行亲缘关系研究,同时对9种滑菇的表型性状进行了相关性分析、主成分分析及聚类分析。研究结果表明,26株供试菌种相似水平在0.76时,聚类分析划分为5个类群。农艺学表型相关性分析表明,各表型性状间的相关系数中达到显著或极显著的性状有6组,说明大部分表型性状间相互影响。主成分分析结果表明,前5个主成分累计贡献率为87.366%,表明这5个主成分能够反映滑菇的9种表型性状的基本特征。从特征值和贡献率来看,菌柄长度、菌菇颜色、菇蕾数量、产量和菌盖厚度是引起滑菇种质表型差异的主要因素。种质资源表型性状分析结果表明,在遗传距离为10时,26份滑菇种质可聚为5个类群。研究结果将为进一步开发利用奠定基础,以期为滑菇菌株新品种选育、优质种源筛选提供依据。Utilizing 26 strains of Pholiota nameko as experimental materials and the ISSR molecular marker technique,we conducted a genetic relationship study on the germplasm resources of Pholiota nameko at both the cellular and molecular levels using 11 ISSR-PCR reaction primers.We also carried out the correlation analysis,principal component analysis,and cluster analysis on the phenotypic traits of nine types of Pholiota nameko.The results showed that the 26 strains of the test strains were classified into five groups at the similarity level of 0.76.The agronomic phenotypic correlation analysis showed that there were six groups of traits with significant or highly significant correlation coefficients,suggesting that most phenotypic traits influence each other.The principal component analysis showed that the first five principal components accounted for 87.366%of the cumulative contribution rate,indicating that these five principal components can reflect the basic characteristics of the nine phenotypic traits of the Pholiota nameko.From the perspective of characteristic values and contribution rates,we found that mushroom stem length,mushroom color,the number of mushroom buds,yield,and cap thickness are the main factors causing phenotypic differences in the germplasm of Pholiota nameko.The analysis of phenotypic traits of the germplasm resources indicated that when the genetic distance was set at 10,the 26 test strain be clustered into five groups.Our findings set the stage for future development and utilization,and provide a basis for the selection and breeding of new varieties of Pholiota nameko strains.

关 键 词：滑菇 ISSR 聚类分析 农艺学表型 

分 类 号：S646.16[农业科学—蔬菜学]