铷矿物质抑制小鼠肺癌增殖与转移作用及机制研究  

Study on the effect and mechanism of rubidium minerals in inhibiting the proliferation and metastasis of lung cancer in mice

作　　者：盘家萍 黄映捷 张东洁 苑仁祎坤 范秋梅 高红伟 赵立春[1,2] PAN Jiaping;HUANG Yingjie;HANG Dongjie;YUAN Renyikun;FAN Qiumei;GAO Hongwei;ZHAO Lichun(Science Experiment Center of Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Zhuangyao Pharmaceutical Engineering Technology Research Center,Nanning 530200,China)

机构地区：[1]广西中医药大学科学实验中心,南宁530200 [2]广西壮瑶药工程技术研究中心,南宁530200

出　　处：《长春中医药大学学报》2024年第4期398-403,共6页Journal of Changchun University of Chinese Medicine

基　　金：国家青年岐黄学者人才项目(2020-7);广西高等学校高水平创新团队项目(2019-52);广西中医药大学2022年研究生教育创新计划项目。

摘　　要：目的研究铷矿物质抑制小鼠肺癌增殖与转移的作用及机制。方法小鼠皮下注射Lewis肺癌(Lewis lung cancer,LLC)细胞构造肺癌动物模型,药物干预14 d后,测量小鼠肿瘤大小、血常规、血清与肿瘤中炎症因子以及活性氧(reactive oxygen species,ROS)表达;观察肿瘤、心、肝、脾、肺、肾等组织病理学改变。小鼠尾静脉注射B16-F10细胞构建黑色素瘤肺癌转移模型,检测血常规变化;观察肺湿重及其病理学改变。结果在铷矿物质对肺癌移植瘤小鼠影响的试验中,药物(铷矿物质或吉非替尼)干预14 d后小鼠白细胞(white blood cell,WBC)、淋巴细胞(lymphocyte,Lym)含量均低于模型对照组(P<0.01),且实体瘤体积和重量偏小,肿瘤组织中肿瘤细胞数目减少,细胞核缩小,出现不同程度坏死;心、肝、肺、肾无明显损伤;脾损伤较轻,脾重降低,血清中白细胞介素(interleukin,IL)-6与ROS含量升高,肿瘤坏死因子-α(tumor necrosis factor,TNF-α)含量降低;肿瘤组织中IL-1β、IL-6含量降低,TNF-α含量升高(P<0.05)。在铷矿物质对肺癌转移小鼠影响的试验中,药物(铷矿物质或吉非替尼)干预18 d后小鼠血液Neu和WBC水平均显著降低,肺湿重显著降低(P<0.001),肺转移灶少,能明显抑制炎症反应和恶性黑色瘤的肺转移。结论铷矿物质能够显著抑制小鼠肺癌的增殖和转移,其作用机制可能是通过调节机体免疫,降低炎症因子水平、抑制ROS生成,从而发挥治疗肺癌作用。Objective To investigate the effect and mechanism of rubidium minerals in inhibiting the proliferation and metastasis of lung cancer in mice.Methods Mice were injected subcutaneously with Lewis lung cancer(LLC)cells to construct the animal model of lung cancer.And after 14 days of drug intervention,the size of tumors,blood routine,inflammatory factors in serum and tumor,and reactive oxygen species(ROS)expression in mice were measured;Histopathological changes in tumor,heart,liver,spleen,lung,kidney were observed.Mouse tail vein injection of B16-F10 cells was used to construct melanoma lung cancer metastasis model,and blood routine changes were detected.The lung wet weight and its pathological changes were observed.Results In the experiment on the effect of rubidium minerals on mice transplanted lung cancer,after 14 days of drug(rubidium minerals or Gefitinib)intervention,the contents of white blood cell(WBC)and lymphocyte(Lym)in mice were lower than those in the model control group(P<0.01);And the volume and weight of the solid tumor were relatively small,the number of tumor cells in tumor tissues decreased,the nucleus shrank,and different degrees of necrosis occured;There was no significant damage to the heart,liver,lungs,or kidneys;Spleen injury was mild,spleen weight decreased,serum levels of interleukin,(IL)-6 and ROS increased,and tumor necrosis factor-α(TNF-α)content decreased;In tumor tissue,the levels of IL-1β and IL-6 decreased,and TNF-α content increased(P<0.05).In the experiment on the effect of rubidium minerals on lung cancer metastasis in mice,after 18 days of drugs(rubidium minerals or Gefitinib)intervention,the contents of neutrophils(Neu)and WBC in mice were significantly reduced,lung wet weight was significantly decreased(P<0.001),there were few lung metastases,and inflammatory response and lung metastasis of malignant melanoma were significantly inhibited.Conclusion Rubidium minerals can significantly inhibit the proliferation and metastasis of lung cancer in mice,and its mechanism of actio

关 键 词：非小细胞肺癌 铷矿物质 炎症反应 氧化应激 

分 类 号：R732.2[医药卫生—肿瘤]