强化低温、低碳氮比污水脱氮的优势菌特性  

Characteristics of preponderant bacteria enhancing the nitrate removal from wastewater with low ratio of carbon to nitrogen at low temperature

作　　者：田源 孙传荣 张道杨 李冯辰 刘莹 白也 张军[1] 吴淼男[1] 张多英 TIAN Yuan;SUN Chuanrong;ZHANG Daoyang;LI Fengchen;LIU Ying;BAI Ye;ZHANG Jun;WU Miaonan;ZHANG Duoying(School of Civil Engineering,Heilongjiang University,Harbin 150080,China;Inspection and Test Center,Boli County Market Supervision Administration,Qitaihe 154500,China)

机构地区：[1]黑龙江大学建筑工程学院,哈尔滨150080 [2]勃利县市场监督管理局检验检测中心,七台河154500

出　　处：《黑龙江大学自然科学学报》2024年第1期62-69,共8页Journal of Natural Science of Heilongjiang University

基　　金：黑龙江省自然科学基金资助项目(LH2019E074);国家级大学生创新训练计划项目(202010212029)。

摘　　要：低温、低碳氮比(BOD5/TN)污水的有效脱氮一直是北方城镇污水处理厂亟待解决的难题。对从哈尔滨地区低温(4~6℃)地下水中筛选获得的优势菌Y24-6进行研究,初步鉴定其为假单胞菌属(Pseudomonas),研究发现菌株Y24-6在自养条件下对硝酸盐去除率为(24.00±1.24)%。通过分析Pseudomonas sp.Y24-6对NO_(3)^(-)-N的去除过程,发现其在2 h内对硝酸盐的去除速率最高,为25.91 mg·L^(-1)·h^(-1)。基因组(序列号:JACBXG000000000)分析结果表明,菌株Y24-6通过CsrA、GlnL、GlnK和GlnA信号蛋白调控菌株的碳、氮代谢,且其中存在CO_(2)固定的关键酶基因(ppc和icd),在有机碳源不足时可通过固定环境中的CO_(2)来去除NO_(3)^(-)-N。将菌株Y24-6固定在S3型填料,24 h后每个填料可形成的菌量为(7.3±3.7)mg。用固定化的菌株Y24-6处理BOD5/TN<1、4℃的实际污水,TN的去除率平均为(34.55±0.09)%,对低温低碳氮比污水处理效果显著,本研究结果为寒区城镇污水冬季脱氮提供了参考依据。Nitrogen removal became a common problem for municipal wastewater with low organic carbon(BOD_(5)/TN)at low temperature.The predominant strain Y24-6 was isolated from 4~6℃groundwater in Harbin region.The preliminary identification results show that it is related with the genus of Pseudomonas.The strain Y24-6 is found to remove nitrate with the efficiency of(24.00±1.24)%at autotrophic condition.The NO_(3)^(-)-N removal process was analyzed and the highest nitrate removal appeared at the primary 2 h with the removal rate of 25.91 mg·L^(-1)·h^(-1).The genome(Accession No.:JACBXG000000000)analysis results show that the strain Y24-6 regulates carbon and nitrogen metabolism with CsrA,GlnL,GlnK and GlnA signal regulator.Key CO_(2)fixation genes(ppc and icd)existing in the strain Y24-6 show that it can fix CO_(2)as carbon source to remove NO_(3)^(-)-N as lack of organic carbon.The strain Y24-6 is immobilized on the S3 carriers and(7.3±3.7)mg of bacterial content is developed on carriers after 24 hours'immobilization.The immobilized strain Y24-6 is used to treat real wastewater of BOD_5/TN<1 at 4℃.TN removal efficiency is(34.55±0.09)%,which shows significant effect in low BOD_5/TN wastewater treatment at low temperature.The results obtained in this study propose references for nitrogen removal from municipal wastewater in winter of cold region.

关 键 词：低温 低碳氮比 污水 脱氮 优势菌 

分 类 号：Q89[生物学]