CRIP1在胃癌中的表达及与临床病理指标和预后的关系研究  

Study on the expression of CRIP1 in gastric cancer and its relationship with clinicopathological index and prognosis

作　　者：张琳 李婷[2] Zhang Lin;Li Ting(Xi’an No.9 Hospital,Xi’an Shaanxi Province 710054,China;Xi’an Central Hospital,Xi’an Shaanxi Province 710004,China)

机构地区：[1]西安市第九医院,西安710054 [2]西安市中心医院,西安710054

出　　处：《中华普外科手术学杂志（电子版）》2024年第2期171-175,共5页Chinese Journal of Operative Procedures of General Surgery(Electronic Edition)

基　　金：陕西省2020年科技计划项目(2020SF-367)。

摘　　要：目的探讨胃癌组织中富含半胱氨酸肠蛋白1(CRIP1)的表达及临床意义。方法选取2020年1月至2021年12月收治的60例胃癌患者作为研究对象。通过免疫组织化学法(IHC)和实时荧光定量PCR(qRT-PCR)检测胃癌组织及相应的癌旁正常组织(距癌组织边缘>5 cm)中CRIP1蛋白及mRNA的表达情况,将患者分为高表达组(n=50例)和低表组(n=10例),应用SPSS 20.0软件处理数据,临床病理特征中计量资料采用(x±s)表示,行独立样本t检验;计数资料采用[例(%)]表示,其中等级计数资料行秩和检验,其他计数资料行χ^(2)检验;采用Kaplan-Meier法绘制生存曲线,Log-Rank检验CRIP1不同表达与胃癌患者预后的关系,并绘制ROC曲线,计算AUC和约登指数,确定最佳截断点。P<0.05为差异有统计学意义。结果胃癌组织中CRIP1蛋白的阳性表达率显著高于癌旁正常组织(83.3%vs.31.7%,P<0.05),且CRIP1 mRNA在胃癌组织中表达显著上调(P<0.05)。CRIP1高表达与浸润深度、pTMN分期及淋巴结转移显著相关(P<0.05)。CRIP1高表达组的累积总生存率及累积无病生存率显著低于CRIP1低表达组(48.0%vs.80.0%,42.0%vs.70.0%),差异有统计学意义(Log-Rankχ^(2)=4.008、3.906,P=0.045、0.048)。通过绘制CRIP1 mRNA相对表达水平对胃癌预后预测的ROC曲线,计算AUC值为0.865(95%CI:0.831-0.896),其中CRIP1 mRNA表达水平对胃癌预后预测的最佳截断值为1.94时,灵敏度为88.7%,特异度为76.5%,约登指数为0.652。结论CRIP1在胃癌组织中呈高表达,且CRIP1高表达与胃癌的浸润深度、pTMN分期、淋巴结转移密切相关,其可能参与了胃癌的发生、发展及转移,并对胃癌患者的预后具有一定的预测价值。Objective To investigate the expression and clinical significance of cysteine-rich intestinal protein-1(CRIP1)in gastric cancer.Methods Sixty patients with gastric cancer admitted from January 2020 to December 2021 were selected as the study objects.Immunohistochemical method(IHC)and real-time quantitative fluorescent PCR(qRT-PCR)were used to detect the expression of CRIP1 protein and mRNA in gastric cancer tissue and corresponding adjacent normal tissue(>5 cm from the margin of cancer tissue).SPSS 20.0 software was used to process the data,and the measurement data in clinicopathological characteristics were expressed as(x±s).Independent sample t test;The count data were represented by[cases(%)],in which Rank Sum test was used for rank count data andχ^(2) test was used for other count data.Kaplan-Meier method was used to draw the survival curve,and Log-Rank was used to test the relationship between different expressions of CRIP1 and the prognosis of gastric cancer patients.ROC curve was drawn,AUC and Jorden index were calculated,and the optimal cut-off point was determined.P<0.05 was considered statistically significant.Results The positive expression rate of CRIP1 protein in gastric cancer tissues was significantly higher than that in adjacent normal tissues(83.3%vs.31.7%,P<0.05),and the expression of CRIP1 mRNA in gastric cancer tissues was significantly up-regulated(P<0.05).The high expression of CRIP1 was significantly correlated with invasion depth,pTMN stage and lymph node metastasis(P<0.05).The cumulative overall survival rate and disease-free survival rate in the high expression group of CRIP1 were significantly lower than those in the low expression group(48.0%vs.80.0%,42.0%vs.70.0%),and the difference was statistically significant(Log-Rankχ^(2)=4.008,3.906,P=0.045,0.048).By drawing the ROC curve of CRIP1 mRNA relative expression level to predict the prognosis of gastric cancer,the AUC value was calculated as 0.865(95%CI:0.831-0.896),When the best cut-off value of CRIP1 mRNA expression level was 1.94,

关 键 词：胃肿瘤 富含半胱氨酸肠蛋白1 基因表达 预后 

分 类 号：R735.2[医药卫生—肿瘤]