mir-98-5p、ALKBH1在肝门部胆管癌组织中表达及与临床病理特征的关系  

Expression of mir-98-5p and ALKBH1 in hilar cholangiocarcinoma and their relationship with clinicopathological features

作　　者：乌吉斯古楞 哈斯高娃[2] Wu Jisiguleng;Hasigao Wa(Department of Hepatobiliary Surgery,Affiliated Hospital of Inner Mongolia Medical University,Hohhot Inner Mongolia Autonomous Region 010050,China;District 1 Geriatric Center,Affiliated Hospital of Inner Mongolia Medical University,Hohhot Inner Mongolia Autonomous Region 010050,China)

机构地区：[1]内蒙古医科大学附属医院肝胆外科,呼和浩特010050 [2]内蒙古医科大学附属医院老年医学中心一区,呼和浩特010050

出　　处：《中华普外科手术学杂志（电子版）》2024年第2期184-187,共4页Chinese Journal of Operative Procedures of General Surgery(Electronic Edition)

基　　金：内蒙古自治区自然科学重点项目(NJZZ22654)。

摘　　要：目的分析肝门部胆管癌(HC)组织中微小RNA-98-5p(mir-98-5p)、ALKB同源蛋白1(ALKBH1)表达水平,并探讨其与患者临床病理特征及预后的关系。方法选取2011年6月至2017年6月行根治性手术的96例HC患者癌组织和相对应的癌旁正常组织(≥5 cm)为研究对象。实时荧光定量PCR(qRT-PCR)法检测组织中mir-98-5p、ALKBH1 mRNA表达水平,免疫组织化学法检测组织中ALKBH1表达。用SPSS 25.0对数据进行统计学分析,计量资料以(x±s)表示,行独立样本t检验;计数资料行χ^(2)检验;使用Pearson相关分析HC组织中mir-98-5p与ALKBH1 mRNA表达水平的关系;使用Kaplan-Meier进行生存曲线分析mir-98-5p、ALKBH1与HC患者5年内总生存率的关系。P<0.05为差异有统计学意义。结果与癌旁正常组织相比,HC组织中ALKBH1 mRNA相对表达水平、ALKBH1阳性率较高(P<0.05),mir-98-5p相对表达水平较低(P<0.05);mir-98-5p、ALKBH1与HC患者淋巴结转移、TNM分期相关(P<0.05)。Pearson相关分析显示,HC组织中mir-98-5p与ALKBH1 mRNA表达水平呈负相关(P<0.05)。Kaplan-Meier生存分析显示,mir-98-5p低表达组5年累计生存率为18.8%,低于mir-98-5p高表达组33.3%(P<0.05);ALKBH1阳性组5年累计生存率为14.0%,低于ALKBH1阴性组43.6%(P<0.05)。结论mir-98-5p低表达、ALKBH1高表达与HC患者淋巴结转移、TNM分期及预后相关。Objective The expression levels of microrNA-98-5p(mir-98-5p)and ALKB homologous protein 1(ALKBH1)in hilar cholangiocarcinoma(HC)were analyzed,and the relationship between microrNA-98-5P and clinicopathological features and prognosis of patients was discussed.Methods Cancer tissues and corresponding adjacent normal tissues(≥5 cm)of 96 patients with HC who underwent radical surgery from June 2011 to June 2017 were selected as the study objects.Real-time quantitative fluorescent PCR(qRT-PCR)was used to detect the mRNA expression levels of mir-98-5p and ALKBH1 in all tissues,and immunohistochemistry was used to detect the expression levels of ALKBH1 in tissues.Statistical analysis was performed using SPSS 25.0,the measurement data were expressed as(x±s),independent sample t test was performed,andχ^(2) test was performed for comparison among the count groups.Pearson correlation was used to analyze the relationship between mir-98-5p and ALKBH1 mRNA expression levels in HC tissues.Kaplan-Meier survival curve was used to analyze the relationship between mir-98-5p,ALKBH1 and 5-year overall survival of HC patients.P<0.05 was considered statistically significant.Results The relative expression level of ALKBH1 mRNA and positive rate of ALKBH1 in HC tissues were higher than those in adjacent normal tissues(P<0.05),while the relative expression level of mir-98-5p was lower in HC tissues(P<0.05).mir-98-5p and ALKBH1 were correlated with lymph node metastasis and TNM stage in HC patients(P<0.05).The results of Pearson correlation analysis showed that mir-98-5p was negatively correlated with ALKBH1 mRNA expression in HC tissues(P<0.05).Kaplan-Meier survival analysis showed that the 5-year cumulative survival rate of mir-98-5p low expression group was 18.8%,which was lower than that of mir-98-5p high expression group(33.3%)(P<0.05).The 5-year cumulative survival rate of ALKBH1-positive group was 14.0%,and that of AlkBH1-negative group was 43.6%(P<0.05).Conclusion Low expression of mir-98-5p and high expression of ALKBH1 were c

关 键 词：胆管肿瘤 微小RNA-98-5p ALKB同源蛋白1 临床病理特征 

分 类 号：R735.8[医药卫生—肿瘤]