基于多元曲线分辨的植物中3种光合色素的抗干扰检测  

Anti-Interference Determination of Three Photosynthetic Pigments in Plant System Based on Multivariate Curve Resolution

作　　者：李磊磊 韦克苏 姚晶 黄兰 孙希文 赵二卫 武圣江 陈颐 王德俊 严秀芳 Li Leilei;Wei Kesu;Yao Jing;Huang Lan;Sun Xiwen;Zhao Erwei;Wu Shengjiang;Chen Yi;Wang Dejun;Yan Xiufang(Guizhou Tobacco Company Bijie City Company,Bijie 551700,Guizhou,China;Guizhou Academy of Tobacco Science,Guiyang 550081,Guizhou,China;Bijie Tobacco Company Qianxi Branch Company,Qianxi 551500,Guizhou,China;Baoshan Branch of Yunnan Provincial Tobacco Company,Baoshan 678000,Yunnan,China;College of Tobacco Science,Guizhou University,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州省烟草公司毕节市公司,贵州毕节551700 [2]贵州省烟草科学研究院,贵州贵阳550081 [3]毕节市烟草公司黔西分公司,贵州黔西551500 [4]云南省烟草公司保山市公司,云南保山678000 [5]贵州大学烟草学院,贵州贵阳550025

出　　处：《山地农业生物学报》2024年第2期8-16,共9页Journal of Mountain Agriculture and Biology

基　　金：贵州省烟草公司毕节市公司科技项目(2022520500240187);贵州省烟草公司科技项目(2021XM24);贵州省烟草科学研究院科技项目(GZYKY2021-09);云南省烟草专卖局科技一般项目(2021530000242014);贵州省基础研究(自然科学)项目(ZK[2022]一般126)。

摘　　要：为克服Arnon公式法测定植物体系中叶绿素a和b以及β-胡萝卜素含量的干扰问题。论文基于可见光谱量测,引入多元曲线分辨-交替最小二乘(MCR-ALS)算法提取单分析物信号、使用朗伯-比尔定律计算浓度,建立了一种测定植物体系中叶绿素a和b以及β-胡萝卜素的定量分析方法,将发展的方法应用于烟草工业烘烤及烟叶醇化过程中3种光合色素的抗干扰定量分析,并对弹簧烟杆装烟烘烤及烟夹装烟烘烤的实际体系进行了加标验证实验。研究结果表明,预测得到叶绿素a和b以及β-胡萝卜素的平均相对误差分别为1.9%和0.2%以及1.2%,0.8%和1.5%以及3.6%,实验结果满足实际检测的需求。论文提出的方法可为复杂植物体系中叶绿素a和b以及β-胡萝卜素含量的测定提供一种新的选择。In order to overcome the interference of Arnon formula method in the determination of chlorophyll a,chlorophyll b andβ-carotene content in plant system.In this paper,based on visible spectrum measurement,the multivariate curve resolution-alternating least squares(MCR-AIS)algorithm was introduced to extract the single analyte signal from the mixed signal of the analysis system,and then the Lambert-Beer’s law was used to calculate the concentration,an analysis method with some advantages of rapid,simple,low-cost,anti-interference,accurate quantitative was estimated for the determination of chlorophyll a,chlorophyll b andβ-carotene in complex plant systems,and the proposed method was applied to the practical analysis system of tobacco industrial baking and tobacco aging process.In addition,the standard addition verification experiments were carried out on the actual system of flue-cured tobacco baking with spring tobacco rod loading method and tobacco clip loading method,the average relative errors of chlorophyll a,chlorophyll b andβ-carotene were predicted to be 1.9%,0.2%and 1.2%,0.8%,1.5%and 3.6%,respectively.The experimental results meet the needs of actual detection.The method proposed in this paper could provide a new choice for the determination of chlorophyll a,chlorophyll b andβ-carotene in complex plant system.

关 键 词：MCR-ALS 紫外可见光谱 叶绿素A 叶绿素B Β-胡萝卜素 

分 类 号：S132[农业科学—农业基础科学]