厨余垃圾同步糖化发酵产纤维素乙醇的工艺优化  

Process Optimization of Simultaneous Saccharification andFermentation for Cellulosic Ethanol Production from Kitchen Waste

作　　者：杨军[1,2] 杜晓雯 周正 王敬敬 高天歌 付文斌 YANG Jun;DU Xiaowen;ZHOU Zheng;WANG Jingjing;GAO Tiange;FU Wenbin(School of Environmental and Municipal Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China;Gansu Key Laboratory of Yellow River Water Environment,Lanzhou 730070,China)

机构地区：[1]兰州交通大学环境与市政工程学院,兰州730070 [2]甘肃省黄河水环境重点实验室,兰州730070

出　　处：《兰州交通大学学报》2024年第2期114-120,137,共8页Journal of Lanzhou Jiaotong University

基　　金：甘肃省高校产业支持计划项目(2020c-38);甘肃省重点研发项目计划(20210201-GXC-0145)。

摘　　要：为提高纤维素乙醇产率,以未经腐烂的果皮和蔬菜垃圾作为原料,采用酿酒酵母与纤维素酶作为催化添加剂进行同步糖化发酵,结合单因素实验和正交实验,优化了利用厨余垃圾同步糖化发酵产生纤维素乙醇的工艺条件。结果表明:在单因素实验中,采用SSF(同步糖化发酵)工艺时,纤维素酶酶加量、酵母用量、发酵温度和初始pH等因素均能使乙醇产率升高,差异不显著;在正交试验中,采用SSF工艺时,对乙醇产率影响的因素从强到弱为发酵温度>纤维素酶酶加量>酵母用量,且在纤维素酶酶加量30 mg、酵母用量10 mL、初始pH=5、发酵温度45℃、发酵72 h条件下,乙醇浓度最高,可达6.43 g/L,产率为0.089 g/(L·h)。研究发现,采用SSF工艺,为提高乙醇产率、实现废弃物资源化、缓解能源短缺压力等方面提供了新的方向。In order to improve the cellulosic ethanol yield,the process conditions of cellulosic ethanol production by simultaneous saccharification and fermentation(SSF)of kitichen waste were optimized by using undecayed fruit peels and vegetable wastes as the raw materials,and brewer's yeast and cellulase enzyme are adopted as the catalytic additives in a single factor experiment and orthogonal experiments.The results showed that,in the one way experiment,the cellulase enzyme dosage,yeast dosage,fermentation temperature and initial pH could increase the ethanol production rate when SSF process was used,and the differences were not significant;In orthogonal experiments,when using the SSF process,the factors affecting ethanol yield from strongest to weakest are fermentation temperature>cellulase dosage>yeast dosage.Under the conditions of cellulase dosage of 30 mg,yeast dosage of 10 mL,initial pH of 5,fermentation temperature of 45℃,and fermentation for 72 hours,the highest ethanol concentration reaches 6.43 g/L,with a yield of 0.089 g/(L·h).Research has found that adopting SSF technology provides a new direction for improving ethanol yield,achieving the resource utilization of waste,and alleviating the pressure of energy shortage.

关 键 词：厨余垃圾 纤维素酶 纤维素乙醇 同步糖化发酵 

分 类 号：X705[环境科学与工程—环境工程]