聚苯乙烯纳米塑料对中肋骨条藻的毒性效应  

Toxic effects of polystyrene nanoplastics on Skeletonema costatum

作　　者：张秀丽[1] 甄毓[1,2,3] 张鑫 景宗学[3] 华金来 刘浩源 ZHANG Xiuli;ZHEN Yu;ZHANG Xin;JING Zongxue;HUA Jinlai;LIU Haoyuan(Sanya Oceanographic Institution,Ocean University of China,Sanya 572024,China;Laboratory for Marine Ecology and Environmental Science,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266071,China;Key Laboratory of Marine Environment and Ecology,Ministry of Education,College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China)

机构地区：[1]中国海洋大学三亚海洋研究院,海南三亚572024 [2]青岛海洋科学与技术国家实验室,海洋生态与环境科学功能实验室,山东青岛266071 [3]中国海洋大学,环境科学与工程学院海洋环境与生态教育部重点实验室,山东青岛266100

出　　处：《海洋环境科学》2024年第2期235-242,共8页Marine Environmental Science

基　　金：国家自然科学基金项目(41976133);崂山实验室科技创新项目(LSKJ202203700)。

摘　　要：为研究纳米塑料对海洋微藻的毒性效应,本文选取粒径为0.1μm的聚苯乙烯(polystyrene,PS)纳米塑料作为目标污染物,探讨其不同浓度(0.1 mg/L、1 mg/L和10 mg/L)对中肋骨条藻(Skeletonema costatum)的毒性效应。结果发现,在PS纳米塑料处理下,中肋骨条藻细胞生长受到显著抑制,细胞内活性氧(ROS)水平升高;当PS纳米塑料浓度为10 mg/L时,中肋骨条藻细胞的丙二醛(MDA)含量增加;浓度为0.1 mg/L和1 mg/L的PS纳米塑料会导致中肋骨条藻细胞内超氧化物歧化酶(SOD)活性增高,浓度为10 mg/L的PS纳米塑料则导致藻细胞内SOD活性先升高后降低;细胞内过氧化氢酶(CAT)活性和细胞凋亡率均随着藻细胞培养时间的延长而增加。本研究可为科学评估PS纳米塑料污染对海洋中肋骨条藻的毒性作用提供重要参考。To study the toxic effects of nanoplastics on marine microalgae,polystyrene(PS)nanoplastics with the particle size of 0.1μm in different concentrations(0.1 mg/L,1 mg/L and 10 mg/L)were selected as the pollutants to investigate their toxic effects on Skeletonema costatum in this paper.The results showed that the growth of S.costatum cells was significantly inhibited and the level of intracellular reactive oxygen species(ROS)increased under the treatments of PS nanoplastics.When the concentration of PS nanoplastics was 10 mg/L,the contents of malondialdehyde(MDA)increased in the cells.The intracellular superoxide dismutase(SOD)activities in the cells also increased when the concentrations of PS nanoplastics were 0.1 mg/L and 1 mg/L,but the intracellular SOD activity increased first and then decreased when the concentration of PS nanoplastics was 10 mg/L.The activities of intracellular catalase(CAT)and the rates of cell apoptosis increased with the extension of incubation time.This study can provide help to scientifically assess the toxic effects of PS nanoplastic pollution on S.costatum.

关 键 词：聚苯乙烯 中肋骨条藻 活性氧 抗氧化物酶 细胞凋亡 

分 类 号：X171.5[环境科学与工程—环境科学]