芒柄花黄素对裸鼠人胃癌细胞SGC7901移植瘤生长的影响及其机制  

Effect and mechanism of formononetin on the growth of human gastric cancer cell SGC7901 in nude mice

作　　者：张皓渝 康欣 李斌 张雅军 尹天圣 ZHANG Hao-yu;KANG Xin;LI Bin;ZHANG Ya-jun;YIN Tian-sheng(Department of General Surgery,the First People's Hospital of Shuangliu District,Chengdu,Chengdu 610200,China)

机构地区：[1]成都市双流区第一人民医院普外科,四川成都610200

出　　处：《中国现代普通外科进展》2024年第3期186-190,共5页Chinese Journal of Current Advances in General Surgery

摘　　要：目的 :探究芒柄花黄素(Form onone tin,Form)对注射人胃癌细胞系SGC7901细胞悬液裸鼠的保护作用和具体机制。方法:建模成功后将裸鼠分为对照组,低、中、高浓度(10、20、40 mg/kg)芒柄花黄素处理组。观察皮下肿瘤,称量湿重,计算出肿瘤湿重抑制率及体积抑制率。Western blot法检测β-catenin、p-β-catenin、CyclinD1、CDK4以及p-Akt、Akt、Bcl-2、Bax、Caspase3蛋白的相对表达量;免疫组织化学方法测定各组裸鼠肿瘤组织中β-catenin、p-β-catenin、CyclinD1、CDK4、Caspase 3、Caspase 9蛋白表达情况。结果 :免疫组织化学方法结果显示β-cate nin、p-β-cate nin、CyclinD1、CDK4表达情况随Form干预浓度增加而减弱;Caspase3、Caspase 9表达随Form干预浓度增加而增强。Western blot结果显示β-Catenin、p-β-Catenin、CyclinD1、CDK4以及p-Akt、Akt、Bcl-2等蛋白的相对表达量随Form干预浓度增加而降低;Caspase3、Bax等蛋白的相对表达量随Form干预浓度增加而上升。结论:Form可以通过抑制Wnt/β-catenin信号通路而抑制胃癌细胞的增殖,同时还可以上调Caspase3来促进胃癌细胞的凋亡。Objective:To explore the protective effect and mechanism of Formononetin(Form)on nude mice injected with human gastric cancer cell line SGC7901 cell suspension.Methods:After successful modeling,nude mice were divided into control group,low,medium and high concentration(10,20,40 mg/kg)ononcetin treatment group.The subcutaneous tumor was observed,the wet weight was weighed,and the wet weight inhibition rate and volume inhibition rate were calcuated.The relative expression levels of β-Catenin,p-β-Catenin,CyclinD1,CDK4,p-Akt,Akt,Bcl-2,Bax,Caspase3 and other proteins were detected by Western blot.The expression ofβ-catenin,p-β-catenin,CyclinD1,CDK4,Caspase3 and Caspase9 in tumor tissues of nude mice were determined by immunohistochemical method.Results:Immunohistochemical results showed that the expressions ofβ-catenin,p-β-catenin,CyclinD1 and CDK4 decreased with the increase of Form concentration.The expression of Caspase3 and Caspase9 increased with the increase of Form concentration.Western blot results showed that the relative expression levels ofβ-Catenin,p-β-Catenin,CyclinD1,CDK4,p-Akt,Akt,Bcl-2 proteins decreased with the increase of Form intervention concentration.The relative expression levels of Caspase3,Bax and other proteins increased with the increase of Form intervention concentration.Conclusion:Form can inhibit the proliferation of gastric cancer cells by inhibiting Wnt/β-catenin signaling pathway,and can also up-regulate Caspase3 to promote apoptosis of gastric cancer cells.

关 键 词：芒柄花黄素 人胃癌细胞 裸鼠移植瘤 抗肿瘤效应 信号通路 

分 类 号：R735.2[医药卫生—肿瘤]