枯草芽孢杆菌生产乙偶姻工艺优化及在斑马鱼引诱中的应用  

Optimization of Bacillus subtilis process for the production of ethyl diphosgene and its application in zebrafish luring

作　　者：高银浩 王铂昊轩 张恩豪 陈奕杰 颜廷瑞 毛林静 张娜 楚杰 孟武[1] GAO Yinhao;WANG Bohaoxuan;ZHANG Enhao;CHEN Yijie;YAN Tingrui;MAO Linjing;ZHANG Na;CHU Jie;MENG Wu(State Key Laboratory of Biobased Materials and Green Papermaking,Department of Bioengineering,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250353,China;Institute of Biology,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China;College of Biology and Brewing Engineering,Taishan University,Tai'an 271000,China)

机构地区：[1]齐鲁工业大学(山东省科学院)生物工程学部生物基材料与绿色造纸国家重点实验室,山东济南250353 [2]齐鲁工业大学(山东省科学院)生物研究所,山东济南250014 [3]泰山学院生物与酿造工程学院,山东泰安271000

出　　处：《齐鲁工业大学学报》2024年第2期48-56,共9页Journal of Qilu University of Technology

基　　金：山东省自然科学基金(ZR2020MC203);国家级大学生创新训练项目(202310431148)。

摘　　要：以枯草芽孢杆菌168为代谢研究菌株,优化发酵工艺,包括培养基配方和发酵条件,最终得到在葡萄糖80.0 g/L,酵母提取物10.0 g/L,玉米浆干粉10.0 g/L,尿素5.0 g/L,K 2HPO_(4)10.0 g/L,MgSO_(4)1.0 g/L,MnSO_(4)0.08 g/L,发酵温度37℃,初始pH 6.5,接种密度3%,液体体积40%的发酵条件下,得到质量浓度最高为32.58 g/L的乙偶姻。将乙偶姻添加到斑马鱼饲料中,研究其对斑马鱼的引诱作用。结果表明,在斑马鱼饲料中添加质量分数0.6%的乙偶姻,可以使饲料的吸引指数从2.11±0.09提高到3.13±0.08,吸引指数提高了48.34%,并且添加适当浓度的乙偶姻可以提高斑马鱼肝脏的抗氧化能力和肠道消化道的活性。综上所述,采用枯草芽孢杆菌168发酵工艺生产乙偶姻安全可靠、回收率高,作为斑马鱼诱食剂的效果显著,应用价值高。In this study,Bacillus subtilis 168 was used as a metabolic research strain to optimize the fermentation process,including medium formulation and fermentation conditions,and the final results were obtained under the glucose 80.0 g/L,yeast extract 10.0 g/L,dried corn syrup 10.0 g/L,urea 5.0 g/L,K 2HPO_(4)10.0 g/L,MgSO_(4)1.0 g/L,MnSO_(4)0.08 g/L,fermentation temperature of 37℃,initial pH 6.5,inoculum density of 3%,and liquid volume of 40%of the fermentation conditions,the highest ethyl diphosgene was obtained as 32.58 g/L.The ethyl diphosgene was added to zebrafish feed to study its enticing effect on zebrafish.The results showed that adding 0.6%of ethyl diphosgene to zebrafish feed increased the attraction index of the feed from 2.11±0.09 to 3.13±0.08,which was a 48.34%increase in the attraction index,and that ethyl diphosgene at the appropriate concentration increased the antioxidant capacity of the zebrafish liver and the activity of the intestinal digestive tract.In conclusion,the use of Bacillus subtilis 168 fermentation process for the production of ethyl diphosgene was safe,reliable,with high recovery rate,and it was effective and of high application value as a zebrafish attractant.

关 键 词：乙偶姻 引诱剂 斑马鱼 枯草芽孢杆菌168 发酵优化 

分 类 号：Q815[生物学—生物工程]