吲哚氰绿和羽扇豆醇复合脂质体用于结肠癌细胞光-免疫协同灭活研究  

Photoimmune Synergistic Inactivation of Colon Cancer Cells Using Indocyanine Green and Lupeol Composite Liposomes

作　　者：秦奋 张涵 张澜 姚翠萍[1] 王斯佳 张镇西[1] Qin Fen;Zhang Han;Zhang Lan;Yao Cuiping;Wang Sijia;Zhang Zhenxi(Key Laboratory of Biomedical Information Engineering of Ministry of Education,Institute of Biomedical Photonics and Sensing,School of Life Science and Technology,Xi’an Jiaotong University,Xi’an 710049,Shaanxi,China)

机构地区：[1]西安交通大学生命科学与技术学院,生物医学光子学与传感研究所,生物医学信息工程教育部重点实验室,陕西西安710049

出　　处：《中国激光》2024年第3期127-137,共11页Chinese Journal of Lasers

基　　金：国家自然科学基金(62275212);广东省生物医学光学影像技术重点实验室开放基金(2020B121201010-3);中德科学中心中德合作交流项目(Mobility Programme M-0063)。

摘　　要：结肠癌已成为我国主要癌症发病种类之一,传统的治疗方法难以抑制其转移和复发。免疫疗法虽然可以通过机体免疫系统清除肿瘤组织,但肿瘤组织中的免疫抑制微环境,往往会导致效果不及预期。光学疗法,包括光热疗法(PTT)和光动力疗法(PDT),不仅可以直接诱导肿瘤细胞凋亡和坏死,还能改善肿瘤组织中的免疫抑制环境,从而促进免疫细胞在肿瘤组织中的浸润和活性,提高免疫治疗效果。笔者创新性地利用吲哚氰绿(ICG)介导的光学疗法和天然免疫活性分子羽扇豆醇(Lupeol)对自然杀伤(NK)细胞免疫活性的提升作用实现光-免疫协同激活作用和抗肿瘤效果,通过纳米脂质体将ICG和羽扇豆醇整合得到Lip-Lupeol&ICG,并将其用于结肠癌细胞灭活研究。结果显示:Lip-Lupeol&ICG在通过两次间隔激光照射后可实现PTT和PDT的两次治疗作用,可将结肠癌细胞活性抑制至43.4%;与此同时,包裹的羽扇豆醇释放后可与光学疗法协同激活NK细胞活性,将结肠癌细胞活性进一步抑制至16.7%,为临床结肠癌治疗提供了一种新思路。Objective Colon cancer is a solid tumor with strong immunogenicity that is prone to metastasis in its early stages.Traditional single-treatment methods have limited efficacy;therefore,the development of new,safe,effective treatment strategies has become urgent.Natural killer(NK)cell-mediated immunotherapy can kill tumor cells in a nonspecific antigen manner and has good prospects for the treatment of malignant tumors.However,because of the common immune escape mechanism in malignant tumor tissues,the activity and infiltration of NK cells in tumor tissues are insufficient,making it difficult to effectively eliminate tumor cells.Recently,it was found that photothermal therapy(PTT)and photodynamic therapy(PDT),which induce local hyperthermia or reactive oxygen species in tumor tissues via laser-induced phototherapy,not only directly induce apoptosis and necrosis of tumor cells but also improve the immunosuppressive environment in tumor tissues by inducing immunogenic death of tumor cells.This promotes the infiltration and activity of immune cells,including NK cells,in tumor tissues.As a pure natural edible substance and a potential anticancer molecule in plants,lupeol directly promotes tumor cell apoptosis and NK cell activity,making it easier for NK cells to recognize and eliminate tumor cells.In this study,we investigated the synergistic effect and mechanism of a nanoliposome carrier combined with FDA-approved indocyanine green(ICG)-mediated optical therapy and the natural molecule,lupeol,in enhancing NK cell activity for colon cancer cell inactivation.The results show that Lip-Lupeol&ICG reduced colon cancer cell activity to 59.6%after 20 min of irradiation and 43.4%after 20 min+10 min of irradiation.When NK cells are added after 20 min+10 min of irradiation,the activity decreased to 16.7%,providing a new approach for colon cancer treatment.Methods The use of nanoliposomes as carriers to encapsulate the photosensitizer,ICG,and the natural anticancer product,lupeol,in fruits and vegetables to prepare nanoliposome

关 键 词：医用光学 吲哚氰绿 羽扇豆醇 光动力疗法 光热力疗法 NK细胞免疫疗法 

分 类 号：R735.3[医药卫生—肿瘤]