血栓心脉宁片通过调控TGF-β_(1)和Runx2表达抑制血管平滑肌细胞钙化的研究  

Xue-shuan-xin-mai-ning tablet inhibits calcification of vascular smooth muscle cells via regulating the expressions of TGF-β_(1) and Runx2

作　　者：张晶[1] 邓毅凡 张钊源 刘娟[1] 朱米雪 余吉玲 何胜虎[1] 周玮[3] ZHANG Jing;DENG Yifan;ZHANG Zhaoyuan;LIU Juan;ZHU Mixue;YU Jiling;HE Shenghu;ZHOU Wei(Clinical Medical College of Yangzhou University/Northern Jiangsu People’s Hospital,Yangzhou 225001,Jiangsu,China;The Yangzhou School of Clinical Medicine of Dalian Medical University,Yangzhou 225001,Jiangsu,China;Affiliated Hospital of Yangzhou University,Yangzhou 225001,Jiangsu,China)

机构地区：[1]扬州大学临床医学院/江苏省苏北人民医院,江苏扬州225001 [2]大连医科大学扬州临床医学院,江苏扬州225001 [3]扬州大学附属医院,江苏扬州225001

出　　处：《现代中西医结合杂志》2024年第5期609-613,共5页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：江苏省中医药科技发展计划项目(MS2021077)。

摘　　要：目的探讨血栓心脉宁片是否可通过调控转化生长因子β_(1)(TGF-β_(1))和Runt相关转录因子2(Runx2)表达抑制血管平滑肌细胞的钙化。方法取对数生长期大鼠血管平滑肌细胞,实验分为5组:阴性组细胞加入DMEM培养液培养;钙化组加入β-磷酸甘油(β-GP)作用24 h诱导血管平滑肌细胞钙化;血栓心脉宁低、中、高剂量组先分别加入125 mg/L、250 mg/L、500 mg/L的血栓心脉宁片培养24 h后再给予β-GP作用24 h。采用CCK-8法检测各组细胞活力,酶标仪检测各组细胞中钙含量、碱性磷酸酶(ALP)活性及TGF-β_(1)含量,Western blot法检测细胞中Runx2蛋白表达情况。结果与阴性组比较,钙化组细胞活力明显下降(P<0.05),细胞中钙含量、ALP活性、TGF-β_(1)含量及Runx2蛋白相对表达量均明显升高(P均<0.05);与钙化组比较,血栓心脉宁各组细胞活力均明显提高(P均<0.05),细胞中钙含量、ALP活性、TGF-β_(1)含量及Runx2蛋白相对表达量均明显降低(P均<0.05),且各指标均呈浓度依赖性变化。结论血栓心脉宁片可能通过调控TGF-β_(1)和Runx2的表达,抑制大鼠血管平滑肌细胞的钙化。Objective It is to investigate whether Xue-shuan-xin-mai-ning tablet inhibits the calcification of vascular smooth muscle cells(VSMCs)by regulating the expressions of transforming growth factor β_(1)(TGF-β_(1))and Runt-related transcription factor 2(Runx2).Methods The rat VSMCs at logarithmic growth period were taken and divided into 5 groups:the cells in the negative group were cultured with DMEM culture medium;the cells in the calcification group were cultured with β-GP for 24 h to induce VSMCs calcification;the cells in the low,medium and high dose groups of Xue-shuan-xin-mai-ning tablet were firstly cultured with 125 mg/L,250 mg/L and 500 mg/L Xue-shuan-xin-mai-ning tablet for 24 h,and then cultured withβ-GP for 24 h.The cell viability of each group was detected by CCK-8,the content of calcium,activity of alkaline phosphatase(ALP)and content of TGF-β_(1) in the cells of each group were detected by ELISA,and the expression of Runx2 protein in the cells was detected by Western blot.Results Compared with the negative group,the cell viability of the calcification group was decreased significantly(P<0.05),and the content of calcium,activity of ALP,content of TGF-β_(1) and the expression of Runx2 protein in the cells were increased significantly(all P<0.05);compared with the calcification group,the cell viability of each Xue-shuan-xin-mai-ning tablet group was increased significantly(all P<0.05),and the content of calcium,activity of ALP,content of TGF-β_(1) and the expression of Runx2 protein in the cells were decreased significantly(all P<0.05),and the changes of each index were concentration-dependent.Conclusion Xue-shuan-xin-mai-ning tablet may inhibit the calcification of VSMCs in rats by regulating the expression of TGF-β_(1) and Runx2.

关 键 词：血栓心脉宁片 钙化 血管平滑肌细胞 转化生长因子β_(1) Runt相关转录因子2 

分 类 号：R-332[医药卫生]