苗药榜留丽叶中毒性成分杜鹃醇的提取工艺与含量测定  

Optimization of Extraction Process and Content Determination of Toxic Component Rhododendrol from Folk Medicine Rhododendron Delavayi Franch

作　　者：郭喆平 曾爱 王丽[2] 杨艳[2] 陈发菊 尚兴 李齐激 赵能武[1,2] GUO Zhe-ping;ZENG Ai;WANG Li;YANG Yan;CHEN Fa-ju;SHANG Xing;LI Qi-ji;ZHAO Neng-wu(College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guizhou Guiyang 550025;Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guizhou Guiyang 550014,China)

机构地区：[1]贵州中医药大学药学院,贵州贵阳550025 [2]贵州省中国科学院天然产物化学重点实验室贵州省天然产物高效利用工程研究中心,贵州贵阳550014

出　　处：《广州化工》2023年第23期38-42,共5页GuangZhou Chemical Industry

基　　金：国家自然科学基金(82160807)。

摘　　要：采用RP-HPLC法,以乙腈(A)-水(B)为流动相梯度洗脱,检测波长210 nm,柱温25℃,流速1 mL·min^(-1);在单因素实验基础之上,采用Box-Behnken响应面分析法优化杜鹃醇的最优工艺条件。杜鹃醇在0.1~2.5μg(r=0.9998)内具有良好的线性关系,平均加样回收率为97.90%,RSD=1.38%,榜留丽叶中杜鹃醇含量在7.02~23.23 mg/g间。在最佳工艺条件下:乙醇浓度为90%、料液为1∶50、温度为90℃、提取3次、每次2 h,杜鹃醇含量达8.17 mg/g。本文所筛选的RP-HPLC检测方法可作为苗药榜留丽的质量控制方法。Using RP-HPLC method,using acetonitrile(A)-water(B)as mobile phase gradient elution,detection wavelength of 210 nm,column temperature of 25℃,flow rate of 1 mL·min^(-1),based on single factor experiment,using Box-Behnken response surface analysis method,the optimal process conditions of Rhododendol were optimized.Box-Behnken response surface analysis method was used to optimize the optimal process conditions of Rhododendron on the basis of single factor experiment.The linear range of Rhododendrol was 0.1~2.5μg,the sample recovery rate was 97.90%,and RSD was 1.38%.The best extraction process for Rhododendol was as follows:ethanol concentration was 90%,solid-liquid ratio was 1∶50,extraction temperature was 90℃,extraction times were three times each time was 2 h,under this condition,the rhododendron content was 8.17 mg/g.This RP-HPLC detection method and extraction process provided a reference for the rational use of the folk medicine Rhododendron delavayi Franch.

关 键 词：苗药 榜留丽 毒性成分 杜鹃醇 提取工艺 响应面法 含量测定 

分 类 号：R917[医药卫生—药物分析学]