用于抑制肿瘤细胞生长的叶酸修饰聚多巴胺涂层的氧化铜纳米颗粒  

Folic acid modified copper oxide nanoparticles coated with polydopamine for inhibition of tumor cell growth

作　　者：邓凯夫 郑轶璐 王通 郑之裕 修梦婷 任蓉 VAN CUONG Nguyen 朱利民[1,2] DENG Kaifu;ZHENG Yilu;WANG Tong;ZHENG Zhiyu;XIU Mengting;REN Rong;VAN CUONG Nguyen;ZHU Limin(College of Biological Science and Medical Engineering,Donghua University,Shanghai,China;Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine,Donghua University,Shanghai,China;Faculty of Chemical Engineering,Industrial University of Ho Chi Minh City,Ho Chi Minh,Vietnam)

机构地区：[1]东华大学生物与医学工程学院,上海 [2]东华大学上海纳米生物材料与再生医学工程研究中心,上海 [3]胡志明市工业大学化学工程学院,越南胡志明市

出　　处：《东华大学学报（自然科学版）》2024年第2期59-69,共11页Journal of Donghua University(Natural Science)

基　　金：上海市科学技术委员会资助项目(22520710400,21WZ2501300,20DZ2254900);中国教育部生物医用纺织材料“111工程”(B07024)。

摘　　要：采用沉淀转化法制备出粒径约为10 nm的氧化铜纳米颗粒(CuO NPs),通过盐酸多巴胺(DA HCl)在其表面进行聚合,并通过叶酸(FA)修饰形成CuO@PDA-FA NPs。采用透射电子显微镜、红外光谱仪、纳米粒径电势分析仪、X射线光电子能谱对纳米颗粒的形貌和性质进行表征。结果表明,制备的CuO@PDA-FA NPs粒径约为190 nm。材料降解亚甲基蓝试验和氧化葡萄糖试验证实CuO@PDA-FA NPs具备类过氧化物酶(POD)活性和类葡萄糖氧化酶(GOD)活性。近红外光热转换试验证实CuO@PDA-FA NPs具有良好的光热效应和光热稳定性。体外细胞毒性试验、细胞摄取试验和活性氧生成试验结果表明,CuO@PDA-FA NPs能主动靶向肿瘤细胞,并通过光热治疗(PTT)/化学动力学治疗(CDT)/饥饿治疗(ST)协同效果杀伤肿瘤细胞。Copper oxide nanoparticles(CuO NPs)with the size of about 10 nm were prepared by precipitation transformation method,and CuO@PDA-FA NPs were synthesized by polymerization of dopamine hydrochloride(DA HCl)and modification of folic acid(FA)on its surface.The morphology and properties of the nanoparticles were characterized by transmission electron microscope,infrared spectrometer,nano-particle size potential analyzer and X-ray photoelectron spectroscopy.The results show that CuO@PDA-FA NPs with a particle size of about 190 nm are prepared.The degradation of methylene blue and glucose oxide confirmed that the material has peroxidase(POD)activity and glucose oxidase(GOD)activity.Near-infrared photothermal conversion experiments show that it has good photothermal effect and photothermal stability.The results of in vitro cytotoxicity test,cell uptake test and reactive oxygen generation test show that CuO@PDA-FA NPs can actively target tumor cells and kill tumor cells through the synergistic effect of photothermal therapy(PTT),chemical dynamic therapy(CDT)and starvation therapy(ST).

关 键 词：氧化铜 纳米颗粒 叶酸 聚多巴胺 化学动力学治疗 光热治疗 模拟酶 

分 类 号：R730.5[医药卫生—肿瘤] TB33[医药卫生—临床医学]