蝮蛇特异性抗体的制备及其效价测定  

Preparation and titer determination of specific antibody of Agkistrodon halys

作　　者：朱俊蓉 陆雪玲 黄周 黄东凌 王璠 韦晚霞 刘恋 黄永龙 王威[1] ZHU Junrong;LU Xueling;HUANG Zhou;HUANG Dongling;WANG Fan;WEI Wanxia;LIU Lian;HUANG Yonglong;WANG Wei(Department of Emergency,the First Affiliated Hospital Guangxi Medical University,Nanning City,Guangxi Zhuang Autonomous Region,530021,China;Guangxi University Key Laboratory of Emergency Medicine,Nanning City,Guangxi Zhuang Autonomous Region,530021,China)

机构地区：[1]广西医科大学第一附属医院急诊科,广西南宁530021 [2]广西高校急诊医学重点实验室,广西南宁530021

出　　处：《蛇志》2024年第1期24-26,41,共4页Journal of Snake

基　　金：区域创新合作项目(项目名称:基于虚拟标准化蛇伤患者治疗模型的蛇伤大数据平台研究及示范应用,项目编号:2022YFQ0085)。

摘　　要：目的制备蝮蛇特异性抗体,为进一步研发抗蛇毒血清提供理论依据。方法以蝮蛇蛇毒蛋白作为抗原,通过免疫新西兰兔制备蝮蛇特异性抗体,应用间接ELISA法检测其效价,Western blot检测其特异性。结果成功制备了蝮蛇特异性抗体血清,其效价可达1∶100000,Western blot检测抗体对蝮蛇蛇毒具有专一性。结论本研究成功制备了蝮蛇特异性抗体,推进了抗蝮蛇毒血清的研发。Objective The preparation of specific antibodies to Agkistrodon halys,provided theoretical basis for further research and development of antivenoms.Methods Using the venom protein of Agkistrodon halys as antigen,the specific antibody of Agkistrodon halys was prepared by immunizing New Zealand rabbits.Its titer was detected by indirect ELISA and its specificity was detected by Western blot.Results The specific antibody serum of Agkistrodon halys was successfully prepared,and its titer was 1∶100000.Western blot analysis showed that the antibody was specific to Agkistrodon halys venom.Conclusion This study successfully prepared the specific antibody of Agkistrodon halys and promoted the research and development of anti-Agkistrodon halys venom serum.

关 键 词：蝮蛇蛇毒 抗体制备 效价测定 

分 类 号：R996.3[医药卫生—毒理学]