小鼠青春期前和青春期早期海马颗粒细胞γ-氨基丁酸受体电流的研究  

Experimental study on GABA receptor currents of hippocampal granulosa cells in pre-pubertal and early pubertal mice

作　　者：刘爱丽 田超 谌辉 Liu Aili;Tian Chao;Shen Hui(School of Basic Medicine,Tianjin Medical University,Tianjin 300070,China;School of Biomedical Engineering and Technology,Tianjin Medical University,Tianjin 300070,China)

机构地区：[1]天津医科大学基础医学院,天津300070 [2]天津医科大学生物医学工程与技术学院,天津300070

出　　处：《国际生物医学工程杂志》2024年第1期60-65,共6页International Journal of Biomedical Engineering

基　　金：国家自然科学基金(62273186);天津市教委科研计划(2021KJ260)。

摘　　要：目的探讨青春期前期和青春期早期雌性小鼠海马齿状回颗粒细胞γ-氨基丁酸(GABA)受体电流的变化。方法以雌性小鼠为研究对象,3~4周龄小鼠为青春期前期组(n=6),5~6周龄小鼠为青春期早期组(n=6)。采用全细胞膜片钳技术,记录青春期前后海马颗粒细胞GABA受体自发抑制性突触后电流(sIPSC)、微小抑制性突触后电流(mIPSC)和tonic电流,并分析其变化。结果青春期前期组和青春期早期组小鼠sIPSC的发放频率分别为(2.22±0.12)、(2.30±0.21)Hz,幅值分别为(19.97±2.01)、(23.80±2.86)pA,两组小鼠sIPSC发放频率和幅值比较差异均没有统计学意义(均P>0.05);青春期前期组和青春期早期组小鼠sIPSC发放频率和幅值的累积概率比较差异均无统计学意义(均P>0.05)。青春期前期组和青春期早期组小鼠mIPSC的发放频率分别为(0.87±0.08)、(2.15±0.21)Hz,幅值分别为(12.51±0.11)、(29.67±0.19)pA,较青春期前期相比,青春期早期小鼠海马颗粒细胞GABA受体mIPSC发放频率升高(P<0.001),幅值增大(P<0.001)。青春期前期组和青春期早期组小鼠sIPSC发放频率和幅值的累积概率比较差异均有统计学意义(均P<0.001)。青春期前期组和青春期早期组小鼠GABA受体tonic电流分别为(17.40±1.64)、(24.70±2.81)pA,两组小鼠GABA受体tonic电流比较差异具有统计学意义(P<0.05)。结论青春期早期雌性小鼠颗粒细胞GABA受体电流较青春期前期有所增强,雌性小鼠进入青春期早期时海马颗粒细胞抑制性活动增强。Objective To investigate the changes inγ-aminobutyric acid(GABA)receptor currents of hippocampal dentate gyrus granule cells in prepubertal and early pubertal female mice.Methods Female mice were selected as the study objects;3 to 4-week-old mice were selected as the pre-puberty group(n=6);and 5 to 6-week-old mice were selected as the puberty group(n=6).The whole-cell patch clamp technique was used to record the spontaneous inhibitory post-synaptic current(sIPSC),mini-inhibitory post-synaptic current(mIPSC),and tonic current of hippocampal granulosa cells in the DG region of pre-pubertal and early pubertal female mice,and their changes were analyzed.Results The frequency of sIPSC in the pre-puberty group and puberty group was(2.22±0.12)Hz and(2.30±0.21)Hz,respectively.The amplitude of sIPSC in the pre-puberty group and the puberty group was(19.97±2.01)pA and(23.80±2.86)pA,respectively.The experimental results showed no significant changes in frequency and amplitude of sIPSC of hippocampal granulosa cells in pre-pubertal and early pubertal mice(all P>0.05),and no statistical significance in the cumulative frequency and amplitude of sIPSC between two groups(all P>0.05).The frequency of mIPSC in the pre-puberty group and the puberty group was(0.87±0.08)Hz and(2.15±0.21)Hz,respectively.The amplitude of mIPSC in the pre-puberty group and puberty group was(12.51±0.11)pA and(29.67±0.19)pA,respectively.Compared with the pre-pubertal mice,the frequency and amplitude of mIPSC from hippocampal granulosa cells in early pubertal mice are significantly increased(P<0.001).There was also a significant difference in the cumulative frequency and amplitude of sIPSC between the two groups(P<0.001).The tonic current of the pre-puberty group and puberty group was(17.40±1.64)pA and(24.70±2.81)pA,respectively,and the tonic current in early pubertal mice was significantly higher than that in pre-pubertal mice(P<0.05).Conclusions GABA receptor current is enhanced in early pubertal female mice compared with pre-pubertal females.

关 键 词：青春期 Γ-氨基丁酸受体 抑制性突触电流 

分 类 号：R-332[医药卫生]