不同含糖饮料摄入水平小学生肠道菌群差异  

Differences in gut microbiota among primary school students with different levels of sugar-sweetened beverage consumption

作　　者：张琴[1] 刘雪婷 张可馨 田野 何春雷 王一迪 徐裕杰 单树方 汪晓语 熊静远 成果 何方[1] ZHANG Qin;LIU Xueting;ZHANG Kexin;TIAN Ye;HE Chunlei;WANG Yidi;XU Yujie;SHAN Shufang;WANG Xiaoyu;XIONG Jingyuan;CHENG Guo;HE Fang(West China School of Public Health and West China Fourth Hospital,Sichuan University,Chengdu(610041),Sichuan Province,China;不详)

机构地区：[1]四川大学华西公共卫生学院(四川大学华西第四医院),成都610041 [2]四川大学华西第二医院西部妇幼医学研究院妇儿营养中心 [3]四川大学华西护理学院

出　　处：《中国学校卫生》2024年第3期335-340,345,共7页Chinese Journal of School Health

基　　金：国家重点研发计划项目(2020YFC2006300)。

摘　　要：目的探讨不同含糖饮料摄入水平小学生肠道菌群的差异,为更好地识别儿童健康风险并制定有针对性的健康政策提供科学依据。方法于2022年6月,采用分层整群随机抽样方法选取成都市健康小学生192名,采用膳食频率问卷调查含糖饮料摄入量,依据小学生每日含糖饮料摄入量中位水平分为低摄入组(96名)和高摄入组(96名)。使用16S rRNA高通量测序对两组小学生新鲜粪便标本进行肠道菌群检测,比较两组肠道菌群的多样性和群落结构差异。结果低摄入组小学生含糖饮料摄入量为(21.3±1.6)mL/d,高摄入组摄入量为(269.6±37.3)mL/d。多样性分析结果显示,低摄入组与高摄入组肠道菌群α多样性的可观测的OTU(Observed_otus)指数[298.50(259.75,342.25),305.50(244.25,367.75)],测序深度(Goods_coverage)指数[1.00(1.00,1.00),1.00(1.00,1.00)],Chao指数[304.18(260.75,348.78),305.88(245.68,370.88)],香农(Shannon)指数[5.88(5.29,6.45),5.71(4.89,6.28)],辛普森(Simpson)指数[0.95(0.91,0.97),0.94(0.88,0.97)]差异均无统计学意义(Z值分别为-0.64,-0.76,-0.54,-1.76,-1.67,P值均>0.05);两组肠道菌群的β多样性差异无统计学意义(R^(2)=0.006,P>0.05)。在菌属水平,低摄入组中布劳特氏菌属[0.033(0.018,0.055)]、纺锤链杆属[0.009(0.005,0.015)]丰度均高于高摄入组[0.024(0.013,0.041),0.006(0.003,0.011)],差异均有统计学意义(Z值分别为-2.52,-2.81,P值均<0.05)。LEfSe分析显示两组菌群的组间差异主要体现在布劳特氏菌属、纺锤链杆属及八叠球菌属(LDA值分别为3.56,3.12,3.53,P值均<0.05)。结论不同含糖饮料摄入水平的小学生肠道菌群多样性和整体结构差异不大,但菌属水平存在物种差异,可为识别小学生的健康风险,制定健康对策提供科学依据。Objective To explore the differences in the gut microbiota of primary school students with different levels of sugarsweetened beverage intake,so as to provide scientific evidence for better identification of health risks in children and the development of targeted health policies.Methods In June 2022,a total of 192 healthy primary school students from Chengdu were selected using a stratified cluster random sampling method.The sugar-sweetened beverage intake was assessed through a dietary frequency questionnaire.Based on the median daily sugar-sweetened beverage intake,primary school students were categorized into a low-intake group(n=96)and a high-intake group(n=96).The gut microbiota in fresh fecal samples from the two groups of primary school students was analyzed using 16S rRNA high-throughput sequencing,and the diversity and community structure differences in the gut microbiota were compared.Results Children in the low-intake group had a sugar-sweetened beverage intake of(21.3±1.6)mL/d,while the high-intake group had an intake of(269.6±37.3)mL/d.Diversity analysis results showed that there were no statistically significant differences between the low-intake and the high-intake group in terms ofαdiversity metrics:Observed_otus index[298.50(259.75,342.25),305.50(244.25,367.75)],Goods_coverage index[1.00(1.00,1.00),1.00(1.00,1.00)],Chao index[304.18(260.75,348.78),305.88(245.68,370.88)],Shannon index[5.88(5.29,6.45),5.71(4.89,6.28)]and Simpson index[0.95(0.91,0.97),0.94(0.88,0.97)](Z=-0.64,-0.76,-0.54,-1.76,-1.67,P>0.05).Furthermore,no statistically significant difference was observed inβdiversity between the two groups(R^(2)=0.006,P>0.05).At the genus level,the abundance of Blautia[0.033(0.018,0.055)]and Fusicatenibacter[0.009(0.005,0.015)]were higher in the low-intake group compared to the high-intake group[0.024(0.013,0.041),0.006(0.003,0.011)]and differences were statistically significant(Z=-2.52,-2.81,P<0.05).LEfSe analysis highlighted intergroup differences primarily in Blautia,Fusicatenibacter and Sar

关 键 词：碳水化合物 饮料 摄食行为 胃肠道 细菌 儿童 

分 类 号：R179[医药卫生—妇幼卫生保健] R155.59[医药卫生—公共卫生与预防医学] Q93-34[生物学—微生物学]