上皮性卵巢癌患者血清LncRNA KCNQ1OT1、 miR-506-3p水平变化及临床意义  

Changes and clinical significance of serum LncRNA KCNQ1OT1 and miR-506-3p levels in patients with epithelial ovarian cancer

作　　者：张跃 王倩倩 吕蓓 陆泓[2] ZHANG Yue;WANG Qianqian;LYU Bei;LU Hong(Department of Gynaecology,Wuxi People′s Hospital Affiliated to Nanjing Medical University,Wuxi,Jiangsu 214000,China;Department of Gynaecology,Cancer Hospital of Nantong University,Nantong,Jiangsu 226300,China)

机构地区：[1]南京医科大学附属无锡人民医院妇科,江苏无锡214000 [2]南通大学附属肿瘤医院妇科,江苏南通226300

出　　处：《国际检验医学杂志》2024年第8期940-945,共6页International Journal of Laboratory Medicine

基　　金：江苏省卫生健康委医学科研立项项目(ZDXKA2021038);无锡市卫生健康委科研项目(M202029)。

摘　　要：目的 探讨上皮性卵巢癌(EOC)患者血清长链非编码核糖核酸(RNA)KCNQ1重叠转录物1(LncRNA KCNQ1OT1)、miR-506-3p水平变化。方法 选取2019年3月至2020年12月南京医科大学附属无锡人民医院收治的102例EOC患者作为EOC组,70例良性卵巢肿瘤患者作为良性组,以及体检的60例健康女性作为对照组。分析各组血清LncRNA KCNQ1OT1、miR-506-3p对EOC的诊断价值,以及EOC患者2年生存率、预后影响因素。结果 3组LncRNA KCNQ1OT1、miR-506-3p相对表达量比较差异有统计学意义(P<0.05)。EOC患者LncRNA KCNQ1OT1、miR-506-3p表达与国际妇产科学联盟(FIGO)分期、分化程度、淋巴结转移、腹膜转移有关(P<0.05)。受试者工作特征(ROC)曲线分析显示,LncRNA KCNQ1OT1、miR-506-3p联合诊断EOC的价值较高,ROC曲线下面积(AUC)高于二者单独诊断。LncRNA KCNQ1OT1低表达组2年生存率高于高表达组(P<0.05),miR-506-3p高表达组高于低表达组(P<0.05)。FIGO分期Ⅲ~Ⅳ期、淋巴结转移、腹膜转移及血清LncRNA KCNQ1OT1上调、miR-506-3p下调是预后不良的危险因素(P<0.05)。结论 血清LncRNA KCNQ1OT1在EOC中上调、miR-506-3p下调,二者联合检测对EOC诊断效能较高,与患者不良预后有关,有望成为辅助评估病情和预后的生物学标志物。Objective To investigate the changes and clinical significance of serum long non-coding RNA KCNQ1 overlapping transcript 1(LncRNA KCNQ1OT1)and miR-506-3p levels in patients with epithelial ovarian cancer(EOC).Methods From March 2019 to December 2020,102 patients with EOC admitted to Wuxi People′s Hospital Affiliated to Nanjing Medical University were selected as the EOC group,70 patients with benign ovarian tumor as the benign group,and 60 healthy women who underwent physical examination as the control group.The diagnostic value of serum LncRNA KCNQ1OT1 and miR-506-3p in EOC,as well as the influencing factors of 2-year survival rate and prognosis of patients with EOC were analyzed.Results There were significant differences in the relative expression levels of LncRNA KCNQ1OT1 and miR-506-3p among the three groups(P<0.05).The expressions of LncRNA KCNQ1OT1 and miR-506-3p in EOC patients were correlated with FIGO stage,differentiation degree,lymph node metastasis and peritoneal metastasis(P<0.05).Receiver operating characteristic(ROC)curve analysis showed that the combined diagnosis value of LncRNA KCNQ1OT1 and miR-506-3p was higher,and the area under ROC curve(AUC)was higher than that of lncrNA KCNQ1OT1 and miR-506-3p alone.The 2-year survival rate of the low expression group of LncRNA KCNQ1OT1 was higher than that of the high expression group(P<0.05),and the high expression group of miR-506-3p was higher than that of the low expression group(P<0.05).FIGO stageⅢtoⅣ,lymph node metastasis,peritoneal metastasis,up-regulation of serum LncRNA KCNQ1OT1 and down-regulation of miR-506-3p were risk factors for poor prognosis(P<0.05).Conclusion Serum LncRNA KCNQ1OT1 is up-regulated and miR-506-3p is down-regulated in EOC.The combined detection of the two has high diagnostic efficacy for EOC,which is related to poor prognosis of patients,and is expected to become a biological marker to assist in the assessment of disease and prognosis.

关 键 词：上皮性卵巢癌 长链非编码RNA KCNQ1重叠转录物1 miR-506-3p 预后 

分 类 号：R737.31[医药卫生—肿瘤]