乙醇-水为流动相的HPLC法测定氢氯噻嗪片的含量  

Determination of the content for Hydrochlorothiazide tablets by HPLC with ethanol-water as mobile phase

作　　者：王静霞[1] 魏长勇 余敏灵 WANG Jingxia;WEI Changyong;YU Minling(College of Light Industry and Engineering,Sichuan Technology&Business College,Dujiangyan,Sichuan,611830 P.R.China;SCMPA Key Laboratory of Quality Research of Traditional Chinese Medicine,Leshan Food and Drug Inspection Center,Leshan,Sichuan,614000 P.R.China)

机构地区：[1]四川工商职业技术学院轻工工程学院,四川都江堰611830 [2]乐山市食品药品检验检测中心四川省药品监督管理局中药质量研究重点实验室,四川乐山614000

出　　处：《华西药学杂志》2024年第2期200-202,共3页West China Journal of Pharmaceutical Sciences

基　　金：四川药品监督管理局科技计划项目(2021012)。

摘　　要：目的通过升高柱温降低柱压,建立较大流速的以乙醇-水为洗脱系统的HPLC法来测定氢氯噻嗪片的含量。方法以0.1 mol·L^(-1)磷酸二氢钠(用磷酸调pH3)-乙醇(88:12)为流动相,采用Welch Ultimate C18色谱柱(250 mm×4.6 mm,5μm),柱温40℃,流速1.5 mL·min^(-1),检测波长271 nm。结果0.0527~1.3185μg氢氯噻嗪与峰面积呈良好的线性关系,平均回收率为98.87%。结论所用方法环保、准确,能替代氢氯噻嗪测定的现行HPLC法。OBJECTIVE To determine the content of Hydrochlorothiazide tablets by HPLC method with ethanol-water as the elution system.METHODS 0.1 mol·L^(-1)sodium dihydrogen phosphate(pH3.0)-ethanol(88:12)was used as mobile phase.Welch Ultimate Cis column was used as the chromatographic column.The column temperature was 40 C;the flow rate was 1.5 mL·min^(-1)and the detection wavelength was 271 nm.RESULTS Hydrochlorothiazide injection volume had a good linear relationship with peak area in the range of 0.0527-1.3185μg,and the average recovery was 98.87%.CONCIUSION The method is environmentally friendly,accurate and can replace the current HPLC method for the determination of Hydrochlorothiazide tablets.

关 键 词：洗脱系统 乙醇 氢氯噻嗪 高效液相色谱法 含量测定 绿色环保 

分 类 号：R917[医药卫生—药物分析学]