脂肪酶固定化常用载体及方法的研究进展  

Research Progress on Common Carriers and Methods for Lipase Immobilization

作　　者：陈莉莉 张建国[1] 丛文杰 周化岚[1] 王明轩 李振海 王继国 CHEN Lili;ZHANG Jianguo;CONG Wenjie;ZHOU Hualan;WANG Mingxuan;LI Zhenhai;WANG Jiguo(Institute of Food Science and Engineering,School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;Toroivd Technology Company Limited,Shanghai 200439,China)

机构地区：[1]上海理工大学健康科学与工程学院食品科学与工程研究所,上海200093 [2]天筛(上海)科技有限公司,上海200439

出　　处：《工业微生物》2024年第2期110-123,共14页Industrial Microbiology

基　　金：中国博士后科学基金第71批面上资助(2022M712140);上海市“超级博士后”激励计划(2022472);上海市国际科技合作基金项目(19230742900)。

摘　　要：脂肪酶被广泛应用于生物传感器、精细化工和生物催化等诸多领域,在此情形下脂肪酶固定化是提高其重复利用率和催化效率的重要手段。文章按照微生物属、海洋属、动植物属的分类,分别介绍了脂肪酶的不同来源,重点总结了金属有机框架、介孔氧化硅和凝胶等常用的新型酶固定化载体,并讨论了吸附法、共价交联法、包埋法、复合法等脂肪酶固定化常用方法的优缺点,以期为后续研究提供理论依据和参考。Lipases are widely used in many fields such as biosensors,fine chemicals,and biocatalysis.Immobilization of lipases is an important way to improve their reusability and catalytic efficiency.In this paper,the sources of lipases are introduced based on the classification of microorganisms,marine,animals and plants.Frequetly-used novel enzyme immobilization carriers such as metal-organic frameworks,mesoporous silica and gels are summarized.And the advantages and disadvantages of common methods for lipase immobilization,such as adsorption,covalent cross-linking,embedding and composite method,are discussed,in order to provide theoretical basis and references for the further research.

关 键 词：脂肪酶 固定化 金属有机框架 介孔氧化硅 凝胶 

分 类 号：Q814.2[生物学—生物工程]