氮添加和水分改变对亚热带人工林红壤微生物多样性及群落组成的影响  

Effects of nitrogen addition and moisture change on microbial diversity and community composition in subtropical plantation red soil

作　　者：孙浩钊 刘桂珍 朱玉璘 林雪萍 郭豪 钱笑杰 方熊 SUN Haozhao;LIU Guizhen;ZHU Yulin;LIN Xueping;GUO Hao;QIAN Xiaojie;FANG Xiong(Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation,College of Resources and Environment,Fujian Agriculture and Forestry University,Fuzhou 350000,China;College of Mechanical and Electrical Engineering,Fujian Agriculture and Forestry University,Fuzhou 350000,China;College of Land Resources and Environment,Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]福建农林大学资源与环境学院,土壤环境健康与调控福建省重点实验室,福州350000 [2]福建农林大学机电工程学院,福州350000 [3]江西农业大学国土资源与环境学院,南昌330045

出　　处：《生态科学》2024年第1期94-104,共11页Ecological Science

基　　金：国家自然科学基金项目(42267034);福建省松林改造提升行动重点科技攻关项目(2021FKJ01)。

摘　　要：氮沉降加剧与全球降雨格局改变是全球变化的重要特征,外源氮素的输入及土壤水分条件的变化势必会影响土壤微生物的多样性和群落组成,进而影响地球化学过程。运用Illumina Miseq高通量测序技术,研究氮添加、水分改变及二者交互作用对亚热带人工林红壤细菌、真菌多样性、群落组成的影响及其与土壤理化因子间的关系。结果表明:土壤细菌多样性随水分含量的降低而减少,且氮添加增强了这一抑制作用。施氮后中水分处理土壤真菌Shannon指数显著高于低水分处理。氮添加显著降低Acidobacteria的相对丰度,增加Actinobacteria、Bacteroidetes的相对丰度。土壤Acidobacteria、Bacteroidetes的相对丰度随土壤含水量的升高而增加,Actinobacteria、Firmicutes则相反。干旱加剧了氮添加对Acidobacteria相对丰度的抑制作用,氮水交互处理显著影响Cyanobacteria的相对丰度。而氮添加、土壤水分及二者交互作用对土壤真菌群落组成均无显著影响。冗余分析表明土壤细菌群落组成的变化与土壤含水量、pH、NO_(3)^(–)-N含量、TN含量显著相关,分别解释了土壤细菌群落组成变化的65.6%、26.1%、21.5%、20.7%。研究表明氮添加会改变干旱对土壤细菌多样性和群落组成的影响的强度和方向。The intensification of nitrogen deposition and the change of global rainfall pattern are important features of global change.The input of exogenous nitrogen and the change of soil water conditions will inevitably affect soil microbial diversity and community composition.Illumina Miseq high-throughput sequencing technology was used to study the effects of nitrogen addition,water change and their interaction on bacterial and fungal diversity and community composition of red soil in a modeling subtropical plantation,as well as their relationships with soil properties.The results showed that:soil bacterial diversity decreased with the decrease of water content,and the inhibition effect was enhanced by the addition of nitrogen.The Shannon index of soil fungi under medium water treatment was significantly higher than that under low water treatment.Nitrogen addition significantly decreased the relative abundance of Acidobacteria,while increased the relative abundance of Actinobacteria and Bacteroidetes.In the soil,the relative abundance of Acidobacteria and Bacteroidetes increased with the increase of soil water content,while Actinobacteria and Firmicutes were on the contrary.Drought intensified the inhibition effect of nitrogen addition on the relative abundance of Acidobacteria.Nitrogen addition and soil water interaction significantly affected the relative abundance of Cyanobacteria.But nitrogen addition,soil moisture and their interactions had no significant effects on soil fungal community composition.The results of redundancy analysis showed that the changes of soil bacterial community composition were significantly related to soil water content,pH,NO_(3)^(–)-N content and TN,which accounted for 65.6%,26.1%,21.5%and 20.7%of the changes of soil bacterial community composition,respectively.These results suggest that nitrogen addition may alter the intensity and direction of the effects of drought on soil bacterial diversity and community composition.

关 键 词：氮沉降 土壤含水量 微生物多样性 微生物群落组成 

分 类 号：S714.3[农业科学—林学]