电凝法与线栓法制备大脑中动脉缺血模型在脑卒中后中枢痛研究中的应用对比  

Comparison of Electrocoagulation and Filament Occlusion in Construction of Middle Cerebral Artery Ischemia Model for Central Post-Stroke Pain Study

作　　者：汲晓宇 刘彤彤 张传汉[1] 祝畅[1] 张玥[1] Ji Xiaoyu;Liu Tongtong;Zhang Chuanhan(Department of Anesthesiology and Pain Medicine,Hubei Key Laboratory of Geriatric Anesthesia and Perioperative Brain Health,Wuhan Clinical Research Center for Geriatric Anesthesia,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)

机构地区：[1]华中科技大学同济医学院附属同济医院麻醉学与疼痛医学科,老年麻醉与围术期脑健康湖北省重点实验室,武汉市老年麻醉临床医学研究中心,武汉430030

出　　处：《华中科技大学学报（医学版）》2024年第2期154-160,共7页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.82071246)。

摘　　要：目的采用电凝法与线栓法分别造成小鼠大脑中动脉缺血,建立脑卒中后中枢痛(central post-stroke pain,CPSP)模型,探究更贴近临床的造模方法。方法选择6~8周龄(20~25g)健康雄性C57BL/6小鼠,随机分为空白对照组(Naive组)、电凝组(dMCAO组)和线栓组(tMCAO组),进行不同造模处理。在造模后行Longa神经功能缺陷评分,利用TTC染色评估大脑梗死体积,通过机械性缩足阈值和热缩足潜伏期评估小鼠的疼痛状态,旷场实验评估小鼠的运动功能。结果与Naive组相比,电凝组和线栓组小鼠造模后神经功能缺陷评分均升高(均P<0.01),TTC染色可观察到不同程度的脑缺血(P<0.05,P<0.01);与Naive组相比,电凝组和线栓组在造模后的第7、14、21、28天均表现出机械性痛觉超敏和热痛觉过敏,两组差异无统计学意义;与Naive组相比,电凝组小鼠在造模后第29天的运动功能无显著差异,而线栓组小鼠的运动功能下降(P<0.01)。结论电凝法和线栓法均可诱发脑卒中后中枢痛,但电凝法更贴近CPSP的临床表征,更具复制意义。Objective To induce middle cerebral artery ischemia by electrocoagulation or filament occlusion to construct central post-stroke pain(CPSP)model in mice,and to identify a more valid and producible animal model of CPSP.Methods Male C57BL/6 mice were randomly divided into control(Naive)group,electrocoagulation(dMCAO)group and filament(tMCAO)group.Neurological defects of mice were measured by using the Longa scale.TTC staining was used to evaluate brain infarct volume.Paw withdrawal threshold and thermal withdrawal latency were used to evaluate nociceptive behavior.Open field test was used to assess motor function.Results Compared with Naive group,the neurological defect scores of both dMCAO and tMCAO group were increased.TTC staining showed considerable discrepancies of infarct area between dMCAO and tMCAO group.Compared with Naive group,mice in both dMCAO and tMCAO group developed mechanical allodynia and thermal hyperalgesia on day 7,14,21,28 after surgery.Furthermore,locomotor activity was not altered on day 29 in dMCAO group as compared with the Naive group,while that of tMCAO group was significantly declined.Conclusion Both dMCAO and tMCAO model can lead to pain-related syndrome,but dMCAO model is more reproducible and capable to mimic clinical characters of CPSP.

关 键 词：脑卒中后中枢痛 动物模型 神经病理性疼痛 大脑中动脉栓塞 脑缺血 

分 类 号：R743.3[医药卫生—神经病学与精神病学]