外源性八肽胆囊收缩素对急性胰腺炎的影响及其机制研究  

The effect and mechanism of exogenous cholecystokinin octapeptide on acute pancreatitis

作　　者：郑刚 祁洪军 陈士辉 商爱军 Zheng Gang;Qi Hongjun;Chen Shihui;Shang Aijun(General surgery Department of Dezhou Municipal Hospital,Shandong Dezhou 253000,China)

机构地区：[1]德州市立医院普外科,山东德州253000

出　　处：《临床普外科电子杂志》2024年第1期8-13,共6页Journal of General Surgery for Clinicians(Electronic Version)

摘　　要：目的研究外源性八肽胆囊收缩素(cholecystokininoctopeptide,CCK-8)对急性胰腺炎的影响,探讨CCK-8通过胆碱能抗炎通路抑制炎症的机制。方法采用SD大鼠建立急性胰腺炎模型,将SD大鼠随机分为四组各18只,分别为假手术组、模型组、模型制备联合CCK-8组(CCK-8组)、模型制备联合膈下迷走神经切断及CCK-8组(切断组),各组分别于3、6、9h分批处死SD大鼠,采用酶联免疫吸附测定试剂盒检测大鼠血清白介素(interleukin,IL)-6、肿瘤坏死因子(tumornecrosisfactor,TNF)-α水平,并观察大鼠胰腺组织病理情况。结果假手术组大鼠各时间点血清IL-6浓度较低,模型组大鼠血清IL-6浓度水平在6h时达到1个高峰。模型组大鼠各时间点IL-6浓度水平均分别高于假手术组大鼠对应时间点IL-6浓度水平;CCK-8组大鼠6h、9h的IL-6浓度水平明显低于模型组大鼠对应时间点IL-6浓度水平;切断组大鼠6h及9h点IL-6浓度水平明显高于CCK-8组大鼠对应时间点IL-6浓度水平,差异均有显著性(P<0.05)。假手术组大鼠血清TNF-α在9h时浓度达到1个高峰;模型组大鼠血清TNF-α浓度在6h时达到1个高峰。模型组大鼠各时间点TNF-α的浓度水平均高于假手术组大鼠对应时间点TNF-α浓度水平;CCK-8组大鼠6h及9h点TNF-α浓度水平明显低于模型组大鼠对应时间点TNF-α浓度水平;切断组大鼠6h及9h点TNF-α浓度水平明显高于CCK-8组大鼠对应时间点TNF-α浓度水平,差异均有显著性(P<0.05)。CCK-8组3、6、9h各亚组胰腺镜下改变较模型组轻,切断组3、6、9h各亚组胰腺病理改变较CCK-8组重。假手术组大鼠各时间点胰腺组织胰腺损伤评分较低;模型组大鼠胰腺损伤评分随时间推移,分值逐渐增加,各时间点评分均高于假手术组大鼠对应时间点的胰腺损伤评分(P<0.05);CCK-8组大鼠各时间点胰腺损伤评分呈逐渐增加趋势,各时间点胰腺损伤评分均低于模型组大鼠对应时间点胰腺损伤评Objective To investigate the effect of exogenous cholecystokinin octapeptide(CCK-8)on acute pancreatitis and explore the mechanism of CCK-8 inhibiting inflammation through the cholinergic anti-inflammatory pathway.Method SD rats were used to establish an acute pancreatitis model and randomly divided into four groups of 18 each:sham surgery group,model group,model preparation combined with CCK-8 group(CCK-8 group),model preparation combined with subphrenic vagus nerve transection and CCK-8 group(transection group).SD rats were euthanized in batches at 3,6,and 9 hours in each group,and their serum interleukin-6(IL-6)was detected using an enzyme-linked immunosorbent assay kit tumor necrosis factor(TNF)-αlevels were measured,and the pathological changes of pancreatic tissue in rats were observed.Result The serum IL-6 concentration in the sham surgery group rats was lower at various time points,while the serum IL-6 concentration in the model group rats reached a peak at 6 hours.The IL-6 concentration levels in the model group rats were higher than those in the sham surgery group rats at corresponding time points;The IL-6 concentration levels in CCK-8 group rats at 6h and 9h were significantly lower than those in the corresponding time points of the model group rats;The IL-6 concentration levels at 6h and 9h in the cut off group rats were significantly higher than those at the corresponding time points in the CCK-8 group rats,with significant differences(P<0.05).The concentration of serum TNF-αin sham operated group rats at 9 hours reaches a peak;The serum TNF-αconcentration in model group rats reaches a peak at 6 hours.The concentration levels of TNF-αin model group rats at various time points were higher than the concentration levels of TNF-αin the sham surgery group rats at corresponding time points;The concentration levels of TNF-αin CCK-8 group rats is significantly lower than the concentration levels of TNF-αin model group rats at 6h and 9h;At 6h and 9h points in the cut off group rats,the concentration le

关 键 词：外源性 八肽胆囊收缩素 急性胰腺炎 胆碱能抗炎通路 机制 

分 类 号：R73[医药卫生—肿瘤]