舒胃方调控SCF/c-Kit通路对功能性消化不良大鼠胃排空功能及Cajal间质细胞的影响  

Effects of Shu Gastric Formula modulating SCF/c-Kit pathway on gastric emptying function and interstitial cells of Cajal in rats with functional dyspepsia

作　　者：任春艳 陈月 时召平 张鹏飞 REN Chunyan;CHEN Yue;SHI Zhaoping;ZHANG Pengfei(Department of Pharmacy,Chengde Traditional Chinese Medicine Hospital,Chengde,Hebei,067000,China;Department of Traditional Chinese Medicine of Outpatient,the People's Government of Chengde)

机构地区：[1]承德市中医院药学部,河北承德067000 [2]承德市人民政府机关门诊部中医科

出　　处：《中国中西医结合消化杂志》2024年第4期321-327,共7页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：承德市科学技术研究与发展计划项目(No:202006A062)。

摘　　要：目的探讨舒胃方调控干细胞生长因子(stem cell factor,SCF)/c-Kit通路对功能性消化不良(functional dyspepsia,FD)大鼠胃排空功能及Cajal间质细胞(interstitial cells of Cajal,ICCs)的影响并进行分子对接,分析其作用机制。方法32只SD级大鼠,随机取8只为空白组,另外24只通过多因素应激干预法建立FD大鼠模型,随机分为模型组、莫沙比利组及舒胃方组。光学显微镜观察大鼠胃窦组织;酚红法检测大鼠胃排空率;透射电镜观察大鼠ICCs的超微结构;ELISA法检测大鼠腹主动脉SCF含量;Western Blot检测大鼠胃窦组织SCF、c-Kit蛋白的表达;对舒胃方中所含活性成分进行收集与筛选,并对“有效成分-靶点”拓扑分析所得度值较高的有效成分进行分子对接。结果各组大鼠胃壁组织结构完全,胃黏膜上皮完整,胃腺体结构和排列规整,未见明显炎性细胞浸润。空白组大鼠ICCs超微结构清晰,数量多,体积大,呈圆形、梭形,细胞核占比多,细胞质占比少,线粒体、内质网、核糖体多;模型组大鼠ICCs超微结构改变,数量少,体积小,线粒体肿胀或空泡化;莫沙比利组及舒胃方组大鼠ICCs超微结构改善,数量增多,体积变大,形态改善。与空白组比较,模型组大鼠胃排空率、血清SCF含量及胃窦组织SCF、c-Kit蛋白表达下降(P<0.05);与模型组比较,莫沙必利组与舒胃方组大鼠胃排空率、血清SCF含量及胃窦组织SCF、c-Kit蛋白表达增加(P<0.05)。舒胃方中党参的主要活性成分有21个,炒白术的主要活性成分有7个,枳实的主要活性成分有22个,厚朴的主要活性成分有2个。分子对接结果显示,与关键靶点对接较好的成分有党参中的Daturilin,炒白术中的14-acetyl-12-senecioyl-2E,8Z,10E-atractylentriol,枳实中的nobiletin和厚朴中的Eucalyptol,与SCF、c-Kit的结合能分别为-6.44、-6.87、-5.72、-5.66、-4.31、-5.47、-5.69、-6.04。结论舒胃方可能通过多成分、多靶点调控SCF/c-Kit�Objective To investigate the effects of Shu Gastric Formula modulating the stem cell factor(SCF)/c-Kit pathway on gastric emptying function and interstitial cells of Cajal(ICCs)in functional dyspepsia(FD)rats and molecular docking,and to analyze its mechanism of action.Methods Thirty-two SD-grade rats,8 were randomly taken as the blank group,and the other 24 were randomly divided into the model group,the mosapride group,and the Shu Gastric Formula group through the multifactorial stress intervention method to establish the FD rat model.The rat gastric sinus tissue was observed by light microscope;the gastric emptying rate was detected by phenol red method;the ultrastructure of rat ICCs was observed by transmission electron microscope;the content of serum SCF in the abdominal aorta of the rats was detected by ELISA;the expression of SCF and c-Kit protein in the gastric sinus tissue of the rats was detected by Western Blot;the active ingredients contained in Shu Gastric Formula were also analyzed by Western Blot;and the results were summarized.The active ingredients contained in Shu Gastric Formula were collected and screened,and molecular docking was carried out on the active ingredients with higher degree values obtained from the"active ingredient-target"topology analysis.Results The histological structure of the stomach wall of rats in each group was complete,the epithelium of gastric mucosa was intact,the structure and arrangement of gastric glands were regular,and no obvious inflammatory cell infiltration was seen.The ultrastructure of ICCs in rats of blank group was clear,with high number,large volume,round and pike shape,more nuclei,less cytoplasm,and more mitochondria,endoplasmic reticulum and ribosomes;the ultrastructure of ICCs in rats of model group was altered,with low number,small volume,and mitochondria were swollen or vacuolated;the ultrastructure of ICCs in rats of the mosapride group and the Shu Gastric Formula group was improved,with increased number,larger volume,and improved morphology.increased

关 键 词：分子对接 舒胃方 SCF/c-Kit通路 功能性消化不良 CAJAL间质细胞 

分 类 号：R473.5[医药卫生—护理学]